东沙珊瑚共附生真菌ZH-IV-2的活性成分研究  

Research of the bioactive compounds from coral-associated fungus ZH-IV-2

作　　者：郑玉冰 汤华[2] 宋克义 周雅岚 毛俊琴 张文[1,2] ZHENG Yu-bing;TANG Hua;SOONG Ker-yea;CHOU Ya-lan;MAO Jun-qin;ZHANG Wen(School of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Research Center for Marine Drugs,School of Pharmacy,Second Military Medical University,Shanghai 200433,China;Department of Oceanography,National Sun Yat-sen University,Kaohsiung 80824,China;People's Hospital of Shanghai Pudong District,Shanghai 201200,China)

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]中国人民解放军第二军医大学药学院,上海200433 [3]台湾国立中山大学,台湾高雄80824 [4]上海浦东新区人民医院,上海201200

出　　处：《中国海洋药物》2018年第5期21-28,共8页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(41576157;U1405227);上海市优秀学科带头人计划项目(15XD1504600)资助

摘　　要：目的研究软珊瑚Sarcophyton sp.共附生真菌ZH-IV-2中的活性成分。方法采用正相硅胶柱色谱、反相ODS柱色谱、凝胶Sephadex LH-20柱色谱以及高效液相色谱(HPLC)等分离手段对真菌ZH-IV-2的乙酸乙酯提取物分离纯化;并通过理化性质、波谱学数据等信息结构并文献报道数据鉴定化合物结构;采用羧基荧光素琥珀酰亚胺酯(CFSE)流式细胞法和酒石酸抗性酸磷酸酶(TRAP)染色法体外评价化合物对原代淋巴细胞的免疫调节活性及对髓系巨噬细胞的破骨细胞分化活性。结果分离得到7个已知化合物,结构分别鉴定为:aspergillusidone B(1)及C(2),emeguisin A(3),unguinol(4),diorcinol(5),6-butyl-3-methyl-(3R,3aR,6S,6aS)-perhydrofuro[3,4-b]furan-2,4-dione(6),unguisin A(7)。在体外活性测试中,化合物3在0.5和1μmol/L时对淋巴细胞增殖有明显的促进作用,化合物6在0.5和1μmol/L时对破骨细胞生成有明显的促进作用,结果均具有显著性差异。结论报道化合物4的13 C-NMR核磁数据,并对化合物7核磁数据归属进行校正。首次对化合物3的免疫调节活性及化合物6的破骨前体细胞分化活性进行报道,具有进一步研究价值。Objective To investigate the bioactive chemical constituents of fungus ZH-IV-2 from the soft coral Sarcophyton sp.Methods The EtOAc extract of the fungus ZH-IV-2 was purified by repeated column chromatography on silica gel,Sephadex LH-20,and RP-HPLC.The structures of the compounds were determined based on spectroscopic analysis and comparison with reported data.The compounds were evaluated in vitro for their immunomodulatory activity on primary lymphocytes and osteoclastogenesis inhibitory activity on myeloid macrophages using 5-Carboxy-fluorescein diacetate N-succinimidyl ester (CFSE)and tartrate resistant acid phosphatase (TRAP)staining methods,respectively.Results Seven known compounds were isolated and identified as aspergilluidones B(1)and C(2),emeguisin A (3),unguinol(4),diorcinol (5)and 6-butyl-3-methyl-(3R,3aR,6S,6aS)-perhydrofuro[-3,4-b-]furan-2, 4-dione (6),and unguisin A (7).Compound 3 could significantly promote T cell proliferation at 0.5 and 1μmol/L,while compound 6 could significantly induce osteoclastogenesis at 0.5 and 1μmol/L. Conclusion Compound 4 was reported for its 13C-NMR data.The NMR assignments for compound 7 were corrected.This was the first report of immunomodulatory activity of 3 and the osteoclastogenesis activity of 6.The research might promote further investigations on the metabolites of these kinds.

关 键 词：珊瑚共附生真菌 真菌ZH-IV-2 缩酚酸环醚 环肽 

分 类 号：R914[医药卫生—药物化学]