上海地区不同PM2.5污染过程对炎症应激影响的差异性  

Effects of Different PM_(2.5) Pollution Processes on Inflammatory Stress in Shanghai Area

作　　者：周骥 孙庆华[3] 许建明[1,2] 彭丽 叶晓芳[1,2] 杨丹丹 ZHOU Ji;SUN Qinghua;XU Jianming;PENG Li;YE Xiaofang;YANG Dandan(Yangtze River Delta Center for Environmental Meteorology Prediction and Warning, Shanghai 200030;Shanghai Key Laboratory of Meteorology and Health, Shanghai Meteorological Service, Shanghai 200030;The Ohio State University, Ohio USA,43026)

机构地区：[1]长三角环境气象预报预警中心,上海200030 [2]上海市气象局,上海市气象与健康重点实验室,上海200030 [3]美国俄亥俄州立大学,美国俄亥俄43026

出　　处：《气象》2018年第12期1612-1617,共6页Meteorological Monthly

基　　金：国家自然科学基金项目(416750111);上海市气象局科技开发面上项目(MS201411)共同资助.

摘　　要：为了探讨短时高浓度与相对长时间低浓度下不同的PM_(2.5)污染过程对小鼠急性氧化应激及炎症反应的不同影响,将48只雄性C57BL/6小鼠按体重随机分为6组(n=6),使用人工气候环境暴露仓模拟不同的PM_(2.5)污染过程,分别给予轻度污染组(PM_(2.5)平均浓度为100μg·m^(-3))连续暴露72 h,中度污染组(PM_(2.5)平均浓度为150μg·m^(-3)),连续暴露48 h,重度污染组(PM_(2.5)平均浓度为250μg·m^(-3))连续暴露28. 8 h;每组设置空白对照组。暴露染毒结束后,采用试剂盒法测定C-反应蛋白(CRP)、结构性一氧化氮合酶(cNOS)、同型半胱氨酸(HCY)、白介素-8(IL-8)。低浓度长时间的PM_(2.5)暴露组的CRP、cNOS、IL-8水平显著高于高浓度短时间暴露组,差异性具有统计学意义(P<0. 05或P<0. 01),cNOS的抑制水平为低浓度长时间组显著高于高浓度短时间组,差异性具有统计学意义(P<0. 05或P<0. 01)。结论:相同暴露剂量下PM_(2.5)轻度污染持续72 h的健康损害比PM_(2.5)重度污染持续28. 8 h的对健康损害更大,PM_(2.5)中度污染持续48 h的健康损害次之,应重视长时间轻、中度污染对人体健康的影响,适当调整空气质量的预警标准。This paper is to explore the different anti-oxidant and inflammation effects of short-time high-dose and long-time low-dose fine particular matter(PM2.5) on rats. Forty-eight healthy male C57BL/6 rats were randomly divided into six groups, eight in each. Low-dose group, moderate-dose group and high-dose group were exposed to PM2.5 at 100 μg· m-3, 150 μg · m-3 and 250 μg · m-3 on average, respectively,using the artificial climate environment expose cabinet. Meanwhile, three blank groups were also set. After exposure,C-reactive protein(CRP),constructivenitric oxide synthase(cNOS),homocysteine(HCY),interleukin-8(IL-8) were analyzed. The results showed that the levels of CRP,HCY and IL-8 in long-term low-dose group are significantly higher than those in the short-term high-dose group(P<0. 05, P<0. 01).The inhibition level of cNOS in the low-dose group is also significantly higher than that in the short-term high-dose group(P<0. 05, P<0. 01). The ranking of PM2.5 exposure to health damage effect is low-dose72 h, moderate-dose 48 h and high-dose 28. 8 h when the PM2.5 exposure dose is same.

关 键 词：暴露差异性 PM2.5 健康风险 炎症反应 抗氧化能力 

分 类 号：X513[环境科学与工程—环境工程]