溶血磷脂酸对背根神经髓鞘相关基因Mag和髓鞘形成负性调节转录因子c-Jun表达的影响  

The study of effect and mechanism of lysophosphatidic acid on expression of myelin gene Mag and myelination negative transcription factor c-Jun in dorasl root

作　　者：陈素昌[1] 彭良玉[2] 王喜连[1] 周忠群[1] 唐杰[1] 谢斌[1] 宋西正[1] 邓丁玲 CHEN Su-chang;PENG Liang-yu;WANG Xi-lian;ZHOU Zhong-qun;TANG lie;XIE Bin;SONG Xi-zheng;DENG Ding-ling(Department of Pain Management,the First Affiliated Hospital of Nanhua University,Hengyang 421001,Hunan,China)

机构地区：[1]南华大学附属第一医院疼痛科,湖南衡阳421001 [2]南华大学附属第一医院麻醉科,湖南衡阳421001 [3]南华大学附属第二医院麻醉科,湖南衡阳421001

出　　处：《广东医学》2018年第24期3591-3594,共4页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:81300971)

摘　　要：目的探讨单次鞘内注射溶血磷脂酸(lysophosphatidic acid,LPA)对背根神经(dorsal root,DR)髓鞘相关基因Mag和髓鞘形成的负性调节转录因子c-Jun表达的影响以及其作用机制。方法实验1:鞘内注射LPA (1 nmol),在不同的时间点(1 h、3 h、1 d、2 d、3 d、7 d、14 d)行髓鞘相关基因Mag的实时荧光定量PCR(Real-time PCR)。实验2:鞘内注射LPA(1 nmol),在不同的时间点(1 h、3 h、6 h、24 h、3 d、5 d)行cJun、p-c-Jun的Western blot。实验3:预先30 min给予JNK抑制剂SP600125(0. 5μg)鞘内注射,再给LPA(1 nmol)鞘内注射,在不同的时间点(20、40、60、120、180 min)行c-Jun的Western blot。结果 (1)给予LPA后1 h髓鞘相关基因Mag的mRNA水平明显的降低,一直持续到注射后第7天,但第14天时不明显。(2)给予LPA(1 nmol)鞘内注射之后1 h,c-Jun明显升高,一直持续到第3天,但第5天不明显; p-c-Jun也在加LPA后1 h明显升高,但只持续到6 h,24 h不明显。(3)预先30 min给JNK抑制剂SP600125后,不能抑制DR c-Jun的升高。结论在给予LPA之后DR髓鞘相关基因Mag是下调的;在给予LPA之后髓鞘形成的负性调节转录因子c-Jun和p-c-Jun都是升高的,但c-Jun不是通过经典的JNK-c-Jun通路发挥作用。Objective To investigate the effects and mechanism of lysophosphatidic acid (LPA) on expression ofmyelin gene Mag and myelination negative regulator transcription factor c-Jun expression in dorsal root (DR).MethodsExperiment 1.Giving the treatment of LPA (1 nmol i.t.) to the male C56 mice,the Real-time PCR of Mag wereperformed at different time points (1 h,3 h,Day 1,Day 3,Day 7,and Day 14 post -injection). Experiment 2.GivingLPA (1 nmol i.t.),treatment,Western blot of c-Jun and p- c-Jun were performed at different time points (1 h,3 h,6 h,24 h,Day 3,and Day 5 post-injection).Experiment 3.Adding JNK inhibitor SP600125 (0.5 μg) 30 min prior toLPA treatment in the DR culture,Western blot of c-Jun and p-c-Jun were performed at different time points (20 min,40 min,60 min,120 min and 180 min post-injection).Results The expression of mRNA level of Mag was significantly reduced from 1 h after injection to Day 7,but it was not significant on Day 14.Western blot analysis showed the expression of c-Jun was significantly up-regulated 1 h after injection,it maintained on Day 3 and but not significant till Day5.Western blot analysis also showed the expression of p-c-Jun was significantly up-regulated 1 h after injection,butit only lasted till 6 h after injection and not significant 24 h after injection.Adding JNK inhibitor SP600125 30 min priorto LPA treatment could not reduce DR c-Jun and p-c-Jun up-regulation-Conclusion The expression of myelin-associated gene Mag is down-regulated after LPA intervention.The transcriptional factors c-Jun and p-c-Jun are obviously increased and but not mediated through the classical JNK pathway.

关 键 词：溶血磷脂酸 背根神经 MAG C-JUN p-c-Jun JNK 

分 类 号：R741[医药卫生—神经病学与精神病学]