黄连素对小鼠S-180移植瘤的抑制作用  

Inhibitory effect of berberine on S-180 transplanted tumor in mice

作　　者：杨艳红[1] 张娜 陈雪艳 陈娟 张俊辉 雷自立 张巨峰 Yanhong Yang;Na Zhang;Xueyan Chen;Juan Chen;Junhui Zhang;Zili Lei;Jufeng Zhang(The First Affiliated Hospital ( School of Clinical Medicine),Guangdong Pharmaceutical University,Guangzhou 510080,China;School of Life Science and Biopharmacology,Guangdong Pharmaceutical University,Guangzhou 510006,China;Institute of Chinese Medicinal Sciences,Guangdong Metabolic Disease Research Center of Integrated Chinese and Western Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China)

机构地区：[1]广东药科大学附属第一医院(临床医学院),广东广州510080 [2]广东药科大学生命科学与生物制药学院,广东广州510006 [3]广东药科大学中医药研究院,广东省代谢病中西医结合研究中心,广东广州510006

出　　处：《生物技术》2018年第6期609-614,共6页Biotechnology

基　　金：国家自然科学基金项目(“EpCAM对肠上皮细胞间连接和细胞极性的调控机制研究”,No.31671520;“复方贞术调脂方通过TLR4/NF-κB通路改善p120-catenin敲低所致肝脏炎症性糖脂代谢紊乱的机制研究”,No.81803912);广东省教育厅特色创新类项目(自然科学)广东药科大学“创新强校工程”资助项目(“尼古丁通过影响肠道菌群导致糖脂代谢异常及复方贞术调脂方干预机制”,No.2017KTSCX102);广东省中医药局科研项目(“利用S180荷瘤小鼠模型对黄连素抑制肿瘤分子机制的初步研究”,No.20182079);广东大学生科技创新培育专项资金(“关于预防截肢的肢体局部简易体外循环机及其效果的研究”,No.pdjhb0261)

摘　　要：[目的]利用S-180荷瘤小鼠及细胞研究黄连素抑制肿瘤的作用及分子机制。[方法]利用免疫组织化学染色、WST1实验、流式细胞术及Real-timePCR等技术,检测黄连素抑制S-180肉瘤细胞的作用及机制。[结果]体内实验结果表明黄连素组生存时间明显延长(P<0.05),抑瘤率为25.19%,肿瘤直径(2.11±0.39)显著小于对照组(2.48±0.20),磷酸化组蛋白H3及CyclinD1阳性细胞数目明显降低(P<0.05)。体外实验结果表明黄连素可以抑制S-180细胞的生长,并诱导凋亡(P<0.05),Real-timePCR结果表明,黄连素组C-myc、CyclinD1、Bcl-2及FAS表达水平降低,Caspase-1及PGC-1α表达水平升高(P<0.05)。[结论]黄连素在体内水平抑制S-180荷瘤小鼠肿瘤的增殖,延长生存时间,在体外水平抑制S-180细胞的生长并诱导凋亡,黄连素通过调控增殖、凋亡及代谢等相关基因发挥抗肿瘤作用。[Objective]To study the inhibitory effect and molecular mechanisms of berberine on S-180 tumor-bearing mice and cells.[Methods]Immunohistochemical staining,WST1 cytotoxicity test,flow cytometry and Real-time PCR were used.[Results]The results of in vivo experiment showed that the survival time of the berberine group was significantly prolonged(P<0.05),the tumor inhibition rate was 25.19%,the average diameter of tumor(2.11 ± 0.39)was significantly lower than that of the control group(2.48 ± 0.20),and the number of phosphorylated histone H3 and Cyclin D1 positive cells was significantly decreased(P<0.05).The results of in vitro experiments showed that berberine could inhibit the growth of S-180 cells and induce apoptosis(P<0.05).Real-time PCR showed that the expressions of C-myc,Cyclin D1,Bcl-2 and FAS were decreased,while the expressions of Caspase-1 and PGC-1a were increased in berberine-treated group(P<0.05).[Conclusion ]Berberine could inhibit the proliferation of S-180 tumors in vivo,and prolong the survival time of S-180 tumor-bearing mice.Berberine could inhibit the growth of S-180 cells and induce apoptosis in vitro.Berberine plays an anti-tumor role by regulating proliferation,apoptosis and metabolism related genes.

关 键 词：黄连素 S-180细胞 荷瘤小鼠 增殖 凋亡 代谢 

分 类 号：R730.52[医药卫生—肿瘤]