柱前衍生HPLC法测定梅花鹿鹿茸和马鹿鹿茸中氨基酸的含量  

Determination of Amino Acids in Sika Deer Antler and Red Deer Velvet by Pre-column Derivatization Chromatography

作　　者：何慧楠 赵大庆 卢姝言 张思雨 刘莉[1] 齐滨[1] HE Hui-nan;ZHAO Da-qin;LU Shu-yan;ZHANG Si-yu;LIU Li;QI Bin(College of Pharmacy,Changchun University of Chinese Medicine,Changehun Jilin 130117,China;Jilin Ginseng Academy,Changchun University of Chinese Medicine,Changehun Jilin 130117,China)

机构地区：[1]长春中医药大学药学院,吉林长春130117 [2]长春中医药大学吉林省人参科学研究院,吉林长春130117

出　　处：《中医药导报》2018年第24期80-84,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家中药标准化项目:鹿茸等3种中药饮片标准化建设(编号:ZYBZH-Y-JL-26);安神补脑液标准化建设(编号:ZYBZH-C-JL-22);吉林省大学生创新创业计划:鹿茸质量标准提升研究

摘　　要：目的:建立测定梅花鹿鹿茸和马鹿鹿茸药材中17种氨基酸含量的方法。方法:采用柱前衍生-高效液相色谱法,色谱柱为Aglient ZORABX SB-Aq C18(250 mm×4.6 mm,5μm),柱温为40℃,检测波长为254 nm,流动相A为0.1 mol/L醋酸钠缓冲液(pH6.5)-乙腈(93∶7),流动相B为乙腈-水(8∶2),流速为1 mL/min。结果:该方法线性关系良好(R2=0.9997),平均加样回收率为100.32%,RSD%为1.17%～4.01%。结论:鹿茸药材的质量受鹿的品种、自然环境、养殖技术等因素的影响。该方法适用于梅花鹿鹿茸和马鹿鹿茸中氨基酸的含量测定,可为质量控制提供保证。Objective:To establish a method for the determination of 17 amino acid contents in sika deer antler and red deer pilose antler.Methods:A pre-column derivatization-high performance liquid chromatography method was used.The column was Aglient ZORABX SB-Aq C18 (250mm×4.6mm,5μm).The column temperature was 40℃,the detection wavelength was 254nm,and the mobile phase A was 0.1mol/L.Sodium acetate buffer (pH 6.5)-acetonitrile (93:7),mobile phase B acetonitrile-water (8:2),flow rate 1mL/min.Results:The method has a good linear relationship (R^2=0.9997),with an average recovery of 100.32% and an RSD of 1.17% to 4.01%.Conclusion:The quality of velvet antler is influenced by deer species,natural environment,and breeding techniques.The method is applicable to the determination of amino acid content in sika deer pilose antler and red deer pilose antler to provide guarantee for the quality control of both.

关 键 词：梅花鹿鹿茸 马鹿鹿茸 氨基酸含量 柱前衍生一高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]