Anti-rheumatoid arthritic effect of volatile components in notopterygium incisum in rats via anti-inflammatory and anti-angiogenic activities  

作　　者：BI Jian-Ping LI PING XU Xi-Xi WANG Ting LI Fei 

机构地区：[1]Shandong Provincial Traditional Chinese Medical Hospital,Affricated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250000,China [2]Center for Drug Safety Evaluation and Research of Zhejiang University,Hangzhou 310000,China [3]State Key Laboratory of Natural Medicines,China Pharmaceutical University,Nanjing 210000,China [4]Key Laboratory for the Research of Aromatic Chinese Medicine in Yunnan,College of Pharmaceutical Science,Yunnan University of Traditional Chinese Medicine,Kunming 650000,China [5]School of Pharmacy,Xinjiang Medical University,Urumqi 830001,China

出　　处：《Chinese Journal of Natural Medicines》2018年第12期926-935,共10页中国天然药物（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.81760745);Major Research Plan of Shandong Province(No.2016GSF202041);Yunnan Provincial Department of Education Research Fund(No.2017ZDX230);Yunnan Innovation Team of Application Research on TCM Theory of Prevention Disease in Yunnan University of TCM(No.2017HC011)

摘　　要：Notopterygium incisum(QH) has been used for the treatment of rheumatoid arthritis(RA), and volatile oils may be its mainly bioactive constituents. The present study was designed to analyze the volatile compounds in QH and to determine the anti-arthritic capacity of Notopterygium volatile oils and the potential mechanism of action. The volatile compounds analysis was conducted by GC-MS. The anti-arthritic capacity test of the volatile oils was conducted on adjuvant-induced arthritis(AIA) rats. The anti-inflammatory property was tested in NO release model in RAW 264.7 cells. Endothelial cells were used to evaluate the anti-proliferative and anti-tube formative effects. 70 compounds were analyzed by GC-MS in the volatile oils. Notopterygium volatile oils weakened the rat AIA in a dose-dependent manner(2, 4, and 8 g crude drug/kg). The NO production by RAW 264.7 was decreased by more than 50% in Notopterygium volatile oils(5, 15, and 45 μg·mL^(-1)) pretreated groups. Notopterygium volatile oils also inhibited EAhy926 cell proliferation and further delayed EAhy926 cell capillary tube formation in a concentration-dependent manner. The anti-NO productive, anti-proliferative, and anti-tube formative effects of Notopterygium volatile oils strongly suggested that the therapeutic effect of QH in AIA might be related to the potent anti-inflammatory and anti-angiogenic capacities of the volatile oils.Notopterygium incisum(QH) has been used for the treatment of rheumatoid arthritis(RA), and volatile oils may be its mainly bioactive constituents. The present study was designed to analyze the volatile compounds in QH and to determine the anti-arthritic capacity of Notopterygium volatile oils and the potential mechanism of action. The volatile compounds analysis was conducted by GC-MS. The anti-arthritic capacity test of the volatile oils was conducted on adjuvant-induced arthritis(AIA) rats. The anti-inflammatory property was tested in NO release model in RAW 264.7 cells. Endothelial cells were used to evaluate the anti-proliferative and anti-tube formative effects. 70 compounds were analyzed by GC-MS in the volatile oils. Notopterygium volatile oils weakened the rat AIA in a dose-dependent manner(2, 4, and 8 g crude drug/kg). The NO production by RAW 264.7 was decreased by more than 50% in Notopterygium volatile oils(5, 15, and 45 μg·mL^(-1)) pretreated groups. Notopterygium volatile oils also inhibited EAhy926 cell proliferation and further delayed EAhy926 cell capillary tube formation in a concentration-dependent manner. The anti-NO productive, anti-proliferative, and anti-tube formative effects of Notopterygium volatile oils strongly suggested that the therapeutic effect of QH in AIA might be related to the potent anti-inflammatory and anti-angiogenic capacities of the volatile oils.

关 键 词：NOTOPTERYGIUM incisum Volatile oil Adjuvant-induced ARTHRITIS NO production Endothelial tube formation 

分 类 号：R965[医药卫生—药理学]