hMLH1、hMSH2、hMSH6在宫颈癌患者中的表达及对其预后的影响  

Expression of hMLH1,hMSH2,hMSH6 in patients with cervical cancer and its effect on prognosis

作　　者：王志英 WANG Zhi Ying(Department of Obstetrics and Gynecology,Ningling County Maternal and Child Health Hospital,Shangqiu 476000,China)

机构地区：[1]河南省商丘市宁陵县妇幼保健院妇产科,476000

出　　处：《中国妇幼卫生杂志》2019年第1期9-14,共6页Chinese Journal of Women and Children Health

摘　　要：目的探讨hMLH1、hMSH2、hMSH6在宫颈癌患者中的表达及对其预后的影响。方法收集2011年1月-2013年1月河南省宁陵县妇幼保健院病理科宫颈癌患者术后石蜡标本90例,采用免疫组织化学法检测宫颈癌组织中hMLH1、hMSH2、hMSH6的表达情况,分析错配修复(mismatch repair,MMR)蛋白表达状态与其临床病理特征及预后之间的关系。结果 MMR表达缺失率为21. 11%(21/90)。MMR表达缺失患者和MMR表达正常患者在分期、分化程度、淋巴结转移方面,差异有统计学意义(P <0. 05),MMR表达缺失患者更多见于中高分化、I期的患者,更难发生淋巴结转移。MMR表达缺失(d MMR)组患者的5年无病生存率为61. 90%(13/21),高于MMR表达正常(p MMR)组患者的40. 58%(28/69),差异有统计学意义(χ2=4. 967,P=0. 026)。dMMR组患者的5年总生存率为76. 19%(16/21),高于p MMR组患者的52. 17%(36/69),差异有统计学意义(χ2=4. 834,P=0. 028)。患者MMR状态、分期、分化程度、淋巴结转移为影响患者无病生存期和总生存期的独立因素。结论宫颈癌患者MMR表达缺失更多见于中高分化、I期的患者,更难发生淋巴结转移; MMR状态为影响宫颈癌患者预后的独立因素。MMR表达缺失患者较MMR表达正常患者预后更好。Objective To investigate the expression of hMLH1,hMSH2,hMSH6 in cervical cancer patients and its effect on prognosis. Methods We collected 90 paraffin-embedded specimens from patients with pathological cervical cancer from January 2011 to January 2013. Immunohistochemistry was used to detect the expression of hMLH1,hMSH2,and hMSH6 in cervical cancer tissues.The relationship between the expression status of mismatch repair(MMR) protein and its clinicopathological features and prognosis was analyzed. Results The missing rate of MMR expression was 21. 11%(21/90). There were significant differences in staging,differentiation and lymph node metastasis between patients withMMR-expressed and normal MMR(P < 0. 05). MMR deletions were more common in patients with moderately-differentiated,stage I,and lymph node metastases were more difficult to occur. The 5-year disease-free survival rate in the deficient mismatch repair(d MMR) group was 61. 90%(13/21),which was significantly higher than that in the(proficient mismatch repair) p MMR group(40. 58%,28/69). The difference was statistically significant(χ^2= 4. 967,P = 0. 026). The 5-year overall survival rate in the d MMR group was 76. 19%(16/21),which was higher than that in the p MMR group [52. 17%(36/69) ]. The difference was statistically significant(χ^2= 4. 834,P = 0. 028). MMR status,staging,differentiation,and lymph node metastasis were independent factors that affected the disease-free survival and overall survival of patients. Conclusions Loss of MMR expression is more common in patients with more moderately-differentiated and stage I patients. and lymph node metastasis is more difficult to occur in patients withMMR deletions. MMR status is an independent factor that affects the prognosis of cervical cancer patients. Patients with missing MMR expression had a better prognosis than patients with normal MMR expression.

关 键 词：错配修复蛋白 宫颈癌 病理特征 预后 

分 类 号：R737.33[医药卫生—肿瘤]