基于多肽和蛋白质分子的纳米生物界面效应及其应用研究  

Peptide-/protein-mediated nano-bio interface and its applications

作　　者：郑永芳[1] 林雨晨 邹宜旻 杨延莲[2] 王琛[1] Yongfang Zheng;Yuchen Lin;Yimin ZOU;Yanlian Yang;Chen Wang(Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,Chinese Academy of Sciences,National Center for Nanoscience and Technology,Beijing 100190,China;Key Laboratory of Standardization and Measurement for Nanotechnology,Chinese Academy of Sciences,National Center for Nanoscience and Technology,Beijing 100190,China)

机构地区：[1]国家纳米科学中心,中国科学院纳米生物效应与安全性重点实验室,北京100190 [2]国家纳米科学中心,中国科学院纳米标准与检测重点实验室,北京100190

出　　处：《科学通报》2018年第35期3783-3798,共16页Chinese Science Bulletin

摘　　要：通过对纳米结构进行表面生物和化学修饰,能够赋予其崭新的界面性质.本文概述了纳米颗粒表面共价修饰和非共价修饰多肽和蛋白质的常用方法,对比了两种修饰方法的优缺点以及构筑纳米生物结构存在的问题;并介绍了多肽和蛋白质界面修饰在改善纳米颗粒生物稳定性、生物分布和靶向性方面的研究工作;在此基础上介绍了纳米生物结构基于抗原-抗体特异性识别在生物检测领域的应用;此外,简单介绍了纳米生物结构在应用过程中所面临的挑战.希望本综述能够有助于科技工作者了解纳米生物结构的构筑方法及其应用方面的进展和挑战,为多肽和蛋白质修饰纳米结构的设计合成提供一些启发和思路.Surface modification can impart nanostructures new interface properties.In this review,we summarize the representative surface modification methods of nanoparticles with peptides and proteins.The biomolecules can be conjugated with nanoparticles by noncovalent and covalent coupling.Both of these approaches have strengths and limitations.Physical adsorption is the most direct and simplest noncovalent method but peptides and proteins adsorbed on solid surface always loss their native structures and biological activity and the behavior of peptides and proteins on surface is still difficult to regulate precisely.A popular noncovalent conjugation with stability and selectivity is via biotin-streptavidin interaction.Covalent attachment is more stable and selective than noncovalent methods.The stability of covalent biomolecule-nanostructure conjugates makes this strategy useful for applications in biological media with other interfering species.1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride(EDC)/N-hydroxysuccinimide(NHS) strategy is widely used for covalent bioconjugation with well-established protocol,but it could lead to hydrolysis problem,crosslinking and uncontrolled heterogeneous orientation.The click chemistry is another covalent strategy with higher selectivity and yield,while it takes an extended reaction time.In addition to considering stability,selectivity,reaction time,and yield mentioned above,covalent bioconjugation should proceed in mild condition and the reagents required should have no interference on properties of biomolecules and nanostructures.In recent years substantial progress has been made in the formation of bionanoconjugates,but there is still no completely reliable approach for biomolecule-nanosturcture conjugation.Heretofore fine-tuning the structure and orientation of peptides and proteins on surface has been challenging.The efforts on improving biostability,biodistribution and targeting of nanoparticles with peptides and proteins functionalization are introduced.Nanostructures a

关 键 词：纳米颗粒 多肽 蛋白质 界面修饰 生物分布 靶向性 生物检测 

分 类 号：Q81[生物学—生物工程]