胶质纤维酸性蛋白、波形蛋白和细胞角蛋白AE1/AE3在脑胶质瘤和脑转移瘤中的表达及其临床意义  

Expressions of glial fibrillary acidic protein, Vimentin and cytokeratin AE1/AE3 in glioma and brain metastases and their clinical significances

作　　者：刘刚[1] 步鹏[2] 韩存芝[3] Liu Gang;Bu Peng;Han Cunzhi(Department of Neurosurgery, Shanxi Provincial Cancer Hospital, Taiyuan 030013, China;Department of Pathology, Shanxi Provincial Cancer Hospital, Taiyuan 030013, China;Department of Etiology, Shanxi Provincial Cancer Institute, Taiyuan 030013, China)

机构地区：[1]山西省肿瘤医院神经外科,太原030013 [2]山西省肿瘤医院病理科,太原030013 [3]山西省肿瘤研究所病因室,太原030013

出　　处：《肿瘤研究与临床》2018年第12期834-837,共4页Cancer Research and Clinic

摘　　要：目的探讨胶质纤维酸性蛋白(GFAP)、波形蛋白(Vimentin)和细胞角蛋白(AE1/AE3)在人脑胶质瘤和脑转移瘤中的表达及其与患者临床病理特征的关系。方法采用免疫组织化学SP法对山西省肿瘤医院2013年2月至2015年2月收治的72例脑胶质瘤和45例脑转移瘤组织中GFAP、Vimentin和AE1/AE3的表达进行检测,并分析三种蛋白表达与患者临床特征、病理参数的关系。结果脑胶质瘤组织中GFAP、Vimentin表达阳性率分别为72.2%(52/72)、73.6%(53/72),高于脑转移瘤组织的13.3%(6/45)、17.8%(8/45),差异均有统计学意义(χ2值分别为54.8和34.6,均P<0.001);而AE1/AE3在脑转移瘤组织中表达阳性率为88.9%(40/45),高于脑胶质瘤组织(6.9%,5/72),差异有统计学意义(χ2=82.2,P<0.001)。GFAP阳性表达与肿瘤病理分级呈负相关(r=-0.57,P<0.05),而Vimentin阳性表达与肿瘤病理分级呈正相关(r=0.62,P<0.05)。GFAP在人脑胶质瘤中的表达与Vimentin呈正相关性(r=0.754,P<0.001)。结论GFAP、Vimentin和AE1/AE3可作为脑胶质瘤与脑转移瘤鉴别诊断的检测指标,特别是对一些组织学诊断较为困难的患者。检测GFAP、Vimentin表达有助于对肿瘤恶性程度及预后进行判断。Objective To investigate the expressions of glial fibrillary acidic protein (GFAP), Vimentin and cytokeratin AE1/AE3 in human glioma and brain metastases and their relationship with clinicopathological features. Methods Immunohistochemistry SP method was used to detect the expression of GFAP, Vimentin and AE1/AE3 in 72 gliomas and 45 brain metastases in Shanxi Provincial Cancer Hospital from February 2013 to February 2015. The relationship between the expressions of three proteins and clinical features and pathological parameters was analyzed. Results The positive rates of GFAP and Vimentin in glioma tissues were 72.2% (52/72) and 73.6% (53/72), respectively, which were higher than those in brain metastatic cancer tissues [13.3% (6/45) and 17.8% (8/45)], and the differences were statistically significant (χ 2 values were 54.8 and 34.6, both P < 0.001), while the positive rate of AE1/AE3 in brain metastases was 88.9% (40/45), which was higher than that in human glioma tissues (6.9%, 5/72), and the difference was statistically significant (χ2 = 82.2, P < 0.001). The positive expression of GFAP was negatively correlated with tumor pathological grade (r = -0.57, P < 0.05), while the positive expression of Vimentin was positively correlated with tumor pathological grade (r = 0.62, P < 0.05). The expression of GFAP in human glioma was positively correlated with Vimentin (r = 0.754, P < 0.001). Conclusions GFAP, Vimentin and AE1/AE3 can be used as markers for the differential diagnosis of glioma and brain metastases, especially for patients with difficult histological diagnosis. Detection of GFAP and Vimentin can help to judge the degree of malignancy and prognosis of the tumors.

关 键 词：脑胶质瘤 脑转移瘤 生物标志物 

分 类 号：R739.4[医药卫生—肿瘤]