New cytosine base editor for plant genome editing  

作　　者：Zhenxiang Li Xiangyu Xiong Jian-Feng Li 

机构地区：[1]State Key Laboratoly of Biocontrol,Guangdong Provincial Key Laboratoly of Plant Resources,School of Life Sciences, Sun Yat-sen University,Guangzhou 510275,China

出　　处：《Science China(Life Sciences)》2018年第12期1602-1603,共2页中国科学（生命科学英文版）

摘　　要：A large number of beneficial agronomic traits in crops are associated with single nucleotide polymorphisms(SNPs)or point mutations(Jiao et al.,2010;Li et al.,2017;Ma et al.,2015).In the past,site-specific point mutations in a target gene can only be achieved through the CRISPR/Cas9 mediated gene replacement via the homology-directed repair(HDR).However,the intrinsically low HDR activity in plant cells and the lack of efficient way to supply abundant HDR templates in plant nucleus have greatly limited the success rate of gene replacement in plants(Ran et al.,2017).In addition,DNA double-strand breaks(DSBs)generated by the Cas9 nuclease prior to HDR may lead to complicated and unexpected genome disturbance(Kosicki et al.,2018).

关 键 词：NEW CYTOSINE DNA double-strand 

分 类 号：Q[生物学]