Mechanisms of Exogenous Nitric Oxide and 24-Epibrassinolide Alleviating Chlorosis of Peanut Plants Under Iron Deficiency  

作　　者：SONG Yiling DONG Yuanjie TIAN Xianyi WANG Wanwan Zhenli HE 

机构地区：[1]College of Resources and Environment, National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer, Shandong Agricultural University [2]Indian River Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida

出　　处：《Pedosphere》2018年第6期926-942,共17页土壤圈（英文版）

基　　金：financially supported by the Shandong Provincial Natural Science Foundation of China (No. ZR-2017MD010);Shandong Province Higher Educational Science and Technology Program (No. J14LF08);Taishan Scholars at Seed Industry Talent Project-Shandong Province Seed Industry Project (No. 2014)

摘　　要：Iron(Fe) is a crucial transition metal for all living organisms including plants; however, Fe deficiency frequently occurs in plant because only a small portion of Fe is bioavailable in soil in recent years. To cope with Fe deficiency, plants have evolved a wide range of adaptive responses from changes in morphology to altered physiology. To understand the role of nitric oxide(NO) and 24-epibrassinolide(EBR) in alleviating chlorosis induced by Fe deficiency in peanut(Arachis hypogaea L.) plants, we determined the concentration of chlorophylls, the activation, uptake, and translocation of Fe, the activities of key enzymes, such as ferric-chelate reductase(FCR),proton-translocating adenosine triphosphatase(H^+-ATPase), and antioxidant enzymes, and the accumulation of reactive oxygen species(ROS) and malondialdehyde(MDA) of peanut plants under Fe sufficiency(100 μmol L^(-1)ethylenediaminetetraacetic acid(EDTA)-Fe) and Fe deficiency(0 μmol L^(-1)EDTA-Fe). We also investigated the production of NO in peanut plants subjected to Fe deficiency with foliar application of sodium nitroprusside(SNP), a donor of NO, and/or EBR. The results showed that Fe deficiency resulted in severe chlorosis and oxidative stress, significantly decreased the concentration of chlorophylls and active Fe, and significantly increased NO production. Foliar application of NO and/or EBR increased the activity of antioxidant enzymes, superoxide dismutase,peroxidase, and catalase, and decreased the ROS and MDA concentrations, thus enhancing the resistance of plants to oxidative stress.Application of NO also significantly increased Fe translocation from the roots to the shoots and enhanced the transfer of Fe from the cell wall fraction to the cell organelle and soluble fractions. Consequently, the concentrations of available Fe and chlorophylls in the leaves were elevated. Furthermore, the activities of H^+-ATPase and FCR were enhanced in the Fe-deficient plants. Simultaneously,there was a significant increase in NO production, especially in the plaIron(Fe) is a crucial transition metal for all living organisms including plants; however, Fe deficiency frequently occurs in plant because only a small portion of Fe is bioavailable in soil in recent years. To cope with Fe deficiency, plants have evolved a wide range of adaptive responses from changes in morphology to altered physiology. To understand the role of nitric oxide(NO) and 24-epibrassinolide(EBR) in alleviating chlorosis induced by Fe deficiency in peanut(Arachis hypogaea L.) plants, we determined the concentration of chlorophylls, the activation, uptake, and translocation of Fe, the activities of key enzymes, such as ferric-chelate reductase(FCR),proton-translocating adenosine triphosphatase(H^+-ATPase), and antioxidant enzymes, and the accumulation of reactive oxygen species(ROS) and malondialdehyde(MDA) of peanut plants under Fe sufficiency(100 μmol L^(-1)ethylenediaminetetraacetic acid(EDTA)-Fe) and Fe deficiency(0 μmol L^(-1)EDTA-Fe). We also investigated the production of NO in peanut plants subjected to Fe deficiency with foliar application of sodium nitroprusside(SNP), a donor of NO, and/or EBR. The results showed that Fe deficiency resulted in severe chlorosis and oxidative stress, significantly decreased the concentration of chlorophylls and active Fe, and significantly increased NO production. Foliar application of NO and/or EBR increased the activity of antioxidant enzymes, superoxide dismutase,peroxidase, and catalase, and decreased the ROS and MDA concentrations, thus enhancing the resistance of plants to oxidative stress.Application of NO also significantly increased Fe translocation from the roots to the shoots and enhanced the transfer of Fe from the cell wall fraction to the cell organelle and soluble fractions. Consequently, the concentrations of available Fe and chlorophylls in the leaves were elevated. Furthermore, the activities of H^+-ATPase and FCR were enhanced in the Fe-deficient plants. Simultaneously,there was a significant increase in NO production, especially in the pla

关 键 词：active iron antioxidant enzyme SUBCELLULAR structure chlorophyll oxidative stress reactive oxygen species sodium NITROPRUSSIDE 

分 类 号：S565.2[农业科学—作物学]