不同处理对刺葡萄愈伤组织花青素和原花青素生物合成的影响  

Effects of Different Treatments on Anthocyanins and Procyanidins Biosynthesis in Spine Grape Callus

作　　者：潘红 赖呈纯[2,3,4] 黄贤贵[2] 范丽华 段长青[3] 李绍振[4] 赖钟雄[1] PAN Hong;LAI Chengchun;HUANG Xiangui;FAN Lihua;DUAN Changqing;LI Shaozhen;LAI Zhongxiong(Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Institute of Agricultural Engineering and Technology,Fujian Academy of Agricultural Sciences /Fujian Key Laboratory of Agricultural Product (Food)Processing,Fuzhou,Fujian 350003,China;College of Food Science &Nutritional Engineering,China Agricultural University,Beijing 100083,China;Beijing Huiyuan Food &Beverage Co.,Ltd.,Beijing 100192,China)

机构地区：[1]福建农林大学园艺植物生物工程研究所,福建福州350002 [2]福建省农业科学院农业工程技术研究所,福建省农产品(食品)加工重点实验室,福建福州350003 [3]中国农业大学食品科学与营养工程学院,北京100083 [4]北京汇源食品饮料有限公司,北京100192

出　　处：《热带作物学报》2018年第12期2404-2409,共6页Chinese Journal of Tropical Crops

基　　金：福建省自然科学基金项目(No.2016J01126);福建省农业科学院科技创新团队项目(No.STIT2017-1-10).

摘　　要：以刺葡萄2个同质不同型的愈伤组织细胞系为材料,研究不同处理因子对其花青素和原花青素合成能力的影响。试验结果表明,刺葡萄DLR细胞系具有高产花青素和原花青素的能力,在培养至45 d时,其花青素与原花青素的含量均达到最高值,分别为113.85μg/g(FW)和3 562.95μg/g(FW);低温、高温、肉桂酸、壳聚糖、紫外线、黑暗和KT等因子,对DLR细胞系花青素和原花青素的累积表现出不同的效应;其中,4℃低温处理下,DLR细胞花青素的积累效果最佳,比对照提高了2.14倍;壳聚糖处理下,原花青素积累效果最佳,是对照的2.84倍。刺葡萄DLW细胞系不具有或极低的花青素和原花青素合成能力,处理因子调控对其作用效果不明显。本研究的结论为进一步进行刺葡萄细胞花青素和原花青素合成调控奠定了基础。Using two homogenous genotype spine grape cell lines with different phenotype as materials,the effects of different treatment factors on the biosynthesis of anthocyanins and proanthocyanidins were investigated in this experiment.The results showed that DLR cell line had the potential of high-yield anthocyanins and proanthoeyanidins.The highest content of anthocyanin and proanthocyanidins,113.85 μg/g (FW)and 3 562.95 μg/g (FW),respectively,was observed cultured 45 days after.The accumulation effects of anthocyanin and proanthocyanidins in the DLR cells were various under different treatment factors,such as low temperature,high temperature,cinnamic acid,chitosan,ultraviolet, dark,KT (kinetin)and etc.The accumulation effect of anthocyanin in DLR cells was optimal cultured at 4℃,which was 2.14 times higher than that of the control.The accumulation effect of proanthocyanidins in DLR cells was optimal under chitosan treatment,which was 2.84 times higher than that of the control.The DLW cell line couldn't synthesize anthocyanins or proanthocyanidius,and the regulation effect of treatment factors was not obvious.The conclusion of this study laid a foundation for the regulation of anthocyanin and proanthocyanidins biosynthesis in spine grape cells.

关 键 词：刺葡萄 愈伤组织 花青素 原花青素 合成调控 

分 类 号：S663.1[农业科学—果树学] TS255.1[农业科学—园艺学]