宁波市奉化区女陛宫颈人乳头瘤病毒18型感染流行变异谱系分析  

Lineage analysis of human papillomavirus type 18 isolates from women with cervical infection in Fenghua district of Ningbo

作　　者：张姝[1] 许国章[1] 倪红霞[1] 李永东[1] Zhang Shu;Xu Guozhang;Ni Hongxia;Li Yongdong(Key Laboratory of Virus Research,Ningbo Municipal Center for Disease Control and Prenvention,Ningbo 3115010, Zhejiang,China)

机构地区：[1]宁波市疾病预防控制中心病毒研究重点实验室,浙江宁波315010

出　　处：《国际流行病学传染病学杂志》2018年第6期401-404,共4页International Journal of Epidemiology and Infectious Disease

基　　金：浙江省医药卫生科技计划(2012ZDA041);宁波市重点科技创新团队计划(2012882018);浙江省医学重点学科“现场流行病学”(07-013).

摘　　要：目的了解宁波市奉化地区妇女感染HPV高危亚型18相关基因E6、E7区基因变异谱系特征。方法采用整群随机抽样方法,收集宁波市奉化区778例妇女的宫颈脱落细胞样本进行HPV分型检测,并对其中HPV18阳性株进行癌相关基因E6、E7区测序和序列分析。结果HPV18检出率为1.3%(10/778),HPV18病毒株E6、E7基因序列分别成功获得11和10条,E6区有1个位点发生碱基颠换(C287G),E7区未发现突变;系统进化树分析显示,奉化地区HPV18变异株主要位于该亚型基因变异谱系A的A1亚系。结论宁波市奉化地区妇女HPV18感染率较低,HPV18病毒株癌相关基因变异较小。Objective To investigate the variant lineage of E6 and E7 oncogenes of HPV type 18(HPV18) isolated from women in Fenghua district of Ningbo.Methods By cluster random sampling,778 samples of cervical exfoliated cells were collected from women in Fenghua district of Ningbo and detected for HPV subtype.The E6 and E7 oncogenes of HPV18 positive strains were sequenced and analyzed.Results The positive rate of HPV18 was 1.3%(10/778).-There were 11 E6 genes and 10 E7 genes successfully sequenced.One base transversion (C287G) was found in E6 gene.There was no variation found in E7 gene.The phylogenetic tree showed that prevalent HPVI8 isolates in Fenghua district of Ningbo belonged to subbreed A1 of lineage A.Conclusions The infection rate and cancer related genovariation of HPV18 are low among women in Fenghua district of Ningbo.

关 键 词：人乳头瘤病毒18 E6 E7 基因 变异 

分 类 号：R737.33[医药卫生—肿瘤]