补阳还五汤吸收入脑及入血成分的UPLC-Q-TOF-MS/MS分析  

UPLC-Q-TOF-MS/MS Analysis of Brain and Blood Absorption Components of Buyang Huanwu Tang After Intragastric Administration

作　　者：沈晓 郑华珠[1] 孟英姣 闫向丽 王圣鑫 余爱明 王利胜[1] SHEN Xiao;ZHENG Hua-zhu;MENG Ying-jiao;YAN Xiang-li;WANG Sheng-xin;YU Ai-ming;WANG Li-sheng(School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]广州中医药大学中药学院

出　　处：《中国实验方剂学杂志》2019年第2期8-13,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81573872);广东省省级科技计划项目(2017KZDXM018)

摘　　要：目的:对大鼠灌胃补阳还五汤提取物后的吸收入脑及入血成分进行分析,为该复方的临床应用提供参考。方法:采用UPLC-Q-TOF-MS/MS对正常大鼠脑组织、血浆和脑缺血再灌注大鼠脑组织、血浆进行分析,色谱条件为流动相甲醇-0. 1%甲酸水溶液梯度洗脱,流速0. 3 mL·min^-1,进样量3μL;质谱条件为电喷雾离子源,正、负离子模式扫描,扫描范围m/z50~1 000。根据保留时间、精确相对分子质量、一级和二级质谱数据等鉴定补阳五汤中的原型成分。结果:补阳还五汤提取物给药后,发现5个化合物能透过血脑屏障进入正常脑组织,鉴定为毛蕊异黄酮苷、芍药内酯苷、芒柄花素-7-O-β-D-葡萄糖苷-6″-O-乙酰基、红花黄色素C和黄芪甲苷;2个化合物能透过血脑屏障进入造模脑组织,鉴定为毛蕊异黄酮苷和芒柄花素-7-O-β-D-葡萄糖苷-6″-O-乙酰基;7个化合物进入正常血浆中,经鉴定为毛蕊异黄酮苷、芍药内酯苷、羟基红花黄色素A等;3个化合物进入造模血浆中,鉴定为毛蕊异黄酮-7-O-β-D-葡萄糖苷-6″-O-乙酰基,6″-O-乙酰基-(6αR,11αR)-9,10-二甲氧基紫檀烷-3-O-β-D-葡糖苷和芒柄花素-7-O-β-D-葡萄糖苷-6″-O-乙酰基。结论:补阳还五汤在正常与脑缺血损伤大鼠中的药效物质基础不尽相同,可为该复方的作用机制研究奠定基础。Objective: To analyze and identify the brain and blood absorption components of rats after intragastric administration of Buyang Huanwu Tang( BYHWT). Method: The brain tissue,plasma of normal rats and the cerebral ischemia-reperfusion rats were analyzed by UPLC-Q-TOF-MS/MS. The prototype components in BYHWT were identified according to retention time,accurate relative molecular weight,primary and secondary mass spectrometry data. Result: After the administration of BYHWT,five compounds were found to enter the normal brain tissue through the blood-brain barrier and identified as calycosin-7-glucoside, albiflorin,formononetin-7-O-β-D-glucoside-6″-O-acetyl,safflower yellow A and astragaloside A;two compounds penetrated the blood-brain barrier and entered modeling brain tissue,and they were identified as calycosin-7-glucoside and formononetin-7-O-β-D-glucoside-6″-O-acetyl;seven compounds entered normal plasma and were identified as calycosin-7-glucoside,albiflorin,hydroxysafflor yellow A, et al;three compounds entered model plasma and identified as calycosin-7-O-β-D-glucoside-6″-O-acetyl, 6″-O-acetyl-( 6αR, 11αR)-9, 10-dimethoxypterocarpan-3-O-β-D-glucoside and formononetin-7-O-β-D-glucoside-6″-O-acetyl. Conclusion: BYHWT has different pharmacological material basis in normal and cerebral ischemia-reperfusion rats.

关 键 词：补阳还五汤 毛蕊异黄酮苷 芍药内酯苷 脑匀浆 芒柄花素-7-O-β-D-葡萄糖苷-6″-O-乙酰基 血浆 原型成分 

分 类 号：R22[医药卫生—中医基础理论] R945[医药卫生—中医学]