基于InDel标记的国内绿豆品种遗传多样性分析及指纹图谱构建  

Genetic Diversity and Fingerprint Analysis of Mungbean Varieties from China Based on InDel Markers

作　　者：李群三 陈景斌 顾和平[2] 袁星星[2] 陈新[2] 崔瑾[1] LI Qun-san;CHEN Jing-bin;GU He-ping;YUAN Xing-xing;CHEN Xin;CUI Jin(College of Life Sciences,Nanjing Agricultural University,Nanjing 210095;Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014)

机构地区：[1]南京农业大学生命科学学院,南京210095 [2]江苏省农业科学院经济作物研究所,南京210014

出　　处：《植物遗传资源学报》2019年第1期122-128,共7页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(31501370);国家食用豆产业技术体系(CARS-08-G15);江苏特粮特经产业技术体系(SXGC【2017】260);国家重点研发计划(2016YFE0203800)~~

摘　　要：绿豆是一种抗逆性强、适应性广的作物。本研究选取19个多态性丰富、条带稳定的InDel标记用于42个绿豆参试品种的遗传多样性分析及指纹图谱构建。19个InDel标记有效等位变异数介于1.0998～1.9955之间,平均为1.750;期望杂合度变幅0.0918～0.5049,平均值是0.421;Nei′s基因多样性指数变幅0.0907～0.4989,平均值为0.416。多态性信息含量变幅0.0866～0.3744,0.25<PIC<0.50为中度多态性位点,本研究的InDel标记平均PIC为0.325。利用UPGMA构建42份绿豆主栽品种的聚类树状图,在遗传相似系数为0.522的阈值处可将42个绿豆品种(系)分为2大类群,遗传分析说明绿豆主栽品种基本已脱离地域的影响。利用19个InDel标记电泳结果,构建了42个绿豆主栽品种的DNA指纹图谱,可以将上述绿豆品种准确区分。本研究中的InDel标记带型简单易读,适宜绿豆DNA指纹图谱构建和绿豆品种真伪鉴定,为绿豆名优品种保护、原产地溯源管理等提供科学依据。Mungbean is a kind of crop with strong resistance, and wide adaptability.The genetic diversity analysis and fingerprint analysis of all samples were detected using 19 polymorphic and stable core markers.The effective alleles of the InDel markers were between 1.100 and 1.996, with an average of 1.750.The expected heterozygosity (He) was between 0.0918~0.5049, with an average of 0.421.The variation of Nei"s gene diversity index was between 0.0907~0.4989, with an average of 0.416.The value of the polymorphic information content (PIC) was between 0.0866~0.3744, and 0.25 < 0.50 was the medium polymorphic.The average PIC content of InDel markers used in this experiment was 0.325.Using UPGMA to construct the clustering tree of 42 varieties of mungbean germplasm resources and the result showed that, 42 mungbean varieties (lines) could be divided into 2 groups while the genetic similarity coefficient is 0.55.Genetic analysis showed that the germplasm resources of the domestic mungbean have been isolated from the effect of regional isolation.By using 19 InDel markers, the DNA fingerprint of 42 cultivars of mungbean was constructed and 42 varieties of mungbean could be distinguished and accurately identified.The bands of InDel markers are easy to read in this study and they are suitable for DNA fingerprinting.Our results provide scientific basis for the identification of varieties, the protection of varieties of mungbean and provenance management.

关 键 词：绿豆 INDEL标记 指纹图谱 遗传多样性分析 

分 类 号：S522[农业科学—作物学]