ERCC1基因在急性缺血性脑卒中的表达及意义  

ERCC1 expression and its significance in acute cerebral ischemic stroke

作　　者：邓小冬[1] 高琴 马英[3] 张波[1] 张伟[1] 木尔扯尔[1] 章丽霞[1] 谢英[1] 金波[1] 刘云[1] DENG Xiaochng;GAO Qin;MAYing;ZHANG Bo;ZHANG Wei;MUER Cher;ZHANG Lixia;XIE Ying;JIN Bo;LIU Yun(Department of Forensic North Sichuan Medical College,Nanchong,Sichuan 637000,China;Department of Internal Medicine,Jialing District People's Hospital,Nanchong,Sichuan 637500,China;Department of Neurology,the Affiliated Hospital of North Sichuan Medical College,Nanchong,Sichuan 637000,China)

机构地区：[1]川北医学院法医学系,四川南充637000 [2]四川省南充市嘉陵区人民医院内科,637500 [3]川北医学院附属医院神经内科,四川南充637000

出　　处：《重庆医学》2019年第1期40-43,共4页Chongqing medicine

基　　金：国家自然科学基金青年科学基金项目(81400966);四川省教育厅自然科学科研基金项目(16ZB0235);四川省科技厅创新苗子工程项目(2017055);四川省南充市市校战略合作科技项目(18SXHZ0172);川北医学院科研发展计划项目(CBY15-A-YB19)

摘　　要：目的检测核酸切除修复交叉互补基因1(ERCC1)在急性缺血性脑卒中(CIS)患者的表达,探讨ERCC1基因与急性CIS的关系。方法收集64例急性CIS患者(卒中组)和70例体检健康者(对照组)外周血样和临床资料。实时荧光定量PCR检测ERCC1基因mRNA在外周血单个核细胞(PBMCs)的相对表达水平,ELISA检测ERCC1蛋白在血浆中的表达水平。结果卒中组ERCC1基因mRNA表达水平显著低于对照组(P<0.05),蛋白表达水平显著高于对照组(P<0.05)。不同卒中类型ERCC1基因mRNA和蛋白表达水平差异均无统计学意义(P>0.05)。ERCC1基因mRNA表达水平与中风史呈负相关(r=-0.409,P=0.013),其蛋白表达水平与之无明显相关性(P>0.05)。结论 ERCC1基因可能参与急性CIS的发生、发展、转归及复发。Objective The expression level of nucleotide excision repair cross complementing 1(ERCC1)was detected in acute cerebral ischemic stroke(CIS)patients for discussing the relationship between ERCC1 and acute CIS.Methods The peripheral blood samples and clinical data were collected from 64 patients with acute CIS(the stroke group)and 70 healthy controls(the control group).The relative expression level of ERCC1 mRNA in peripheral blood mononuclear cells(PBMCs)was detected by real-time quantitative PCR,and the ERCC1 protein level in plasma was detected by enzyme-linked immunosorbent assay(ELISA).Results The relative expression level of ERCC1 mRNA in the stroke group was lower than that in the control group(P<0.05).The protein level was significantly higher than that in the control group(P<0.05).There was no significant difference on ERCC1 mRNA or protein expression level among different stroke types(P>0.05).There was a negative correlation between ERCC1 mRNA and the history of stroke(r=-0.409,P=0.013).However,there was no significant correlation between the protein expression level and the history of stroke(P>0.05).Conclusion ERCC1 might be related to the occurrence,development,prognosis and recrudescence of acute CIS.

关 键 词：卒中 DNA 互补 基因表达 ERCC1 

分 类 号：R743.33[医药卫生—神经病学与精神病学]