胃癌患者化疗药物体外敏感性的相关性研究  

Correlation research of in-vitro chemosensitivity of chemotherapy drugs in gastric cancer

作　　者：赵法之 肖硕萌[1] 赵平[1] 丁志[1] 周祥[1] 唐令超[1] 陈小东[1,2] ZHAO Fazhi;XIAO Shuomeng;ZHAO Ping;DING Zhi;ZHOU Xiang;TANG Lingchao;CHEN Xiaodong(Gastrointestinal Surgery Center,Sichuan Cancer Hospital,Chengdu,Sichuan 610041,China;School of Medicine,University of Electronic Science and Technology of China,Chengdu,Sichuan 610054,China)

机构地区：[1]四川省肿瘤医院胃肠外科中心,成都610041 [2]电子科技大学医学院,成都610054

出　　处：《重庆医学》2019年第1期56-60,共5页Chongqing medicine

基　　金：四川省卫生和计划生育委员会科研课题(18PJ205);四川省科技计划项目(2014FZ0089)

摘　　要：目的探讨胃癌患者临床病理特征、化疗相关基因表达水平与化疗药物敏感性的相互关系。方法收集127例病理确诊并接受手术治疗的胃癌患者基线及手术病理资料,采用分支DNA液相芯片技术及ATP肿瘤化疗敏感性检测(ATP-TCA)法检测胃癌细胞中化疗药物相关基因mRNA表达及不同化疗药物体外化疗敏感指数(CSI)并计算药物化疗敏感性,分析患者临床病理特征、化疗相关基因表达水平与化疗药物敏感性的相关性。结果胃下部肿瘤与切除修复交叉互补基因1(ERCC1)高表达有关(P<0.05),BormannⅠ型患者与3型β微管蛋白(TUBB3)高表达有关(P<0.05),肿瘤平均径线大于5cm与胸苷酸合成酶(TYMS)高表达有关(P<0.01),年龄大于或等于60岁、胃上部肿瘤与奥沙利铂(OX)低CSI、胃中部肿瘤与多西他赛(DOC)低CSI及N分期为2~3期与紫杉醇(PTX)低CSI有关(P<0.05)。化疗相关基因mRNA检测值与相应药物CSI及化疗敏感性未表现出相关性(P>0.05)。结论胃癌化疗药物相关基因与体外敏感性不相关。Objective To investigate the correlation among clinicopathological features,chemotherapyassociated gene expression levels and in-vitro chemosensitivity of chemotherapy drugs in gastric tumor patients.Methods Collected the baseline information and surgicopathological characteristics of 127 gastric cancer patients histologically confirmed and surgically treated.The mRNA expression levels of thymidylate synthase(TYMS),excision repair cross complementation group 1(ERCC1),breast cancer susceptibility gene 1(BRCA1)andβ-tubulin-Ⅲ(TUBB3)in tumor tissue were identified by branched-DNA liquid chip quantitative analysis.Adenosine triphosphate based tumor chemosensitivity assay(ATP-TCA)was performed to indicate the chemosensitivity index(CSI).Chemosensitivity properties of different agents were calculated.Correlations among clinicopathological characters,chemotherapy-associated gene mRNA expression levels and chemosensitivity properties were analyzed.Results Correlation analysis indicated that epigastriectomy was in connection with high expression of ERCC1(P<0.05).Bormann type I was in connection with high expression of TUBB3(P<0.05).Less than or equal to 60 years old,subgastrectomy were in connection with low CSI of oxaliplatin(OX).mesogastric tumor was in connection with CSI of docetaxel(DOC),N stage 2-3 and CSI of paclitaxel had statistical significant difference(P <0.05).Correlations between chemotherapy-assocaited genes mRNA and CSI of corresponding agents showed no statistical correlation(P>0.05).Conclusion There is no correlation between chemotherapy-associated genes expression and in-vitro chemosensitivity in gastric cancer.

关 键 词：胃肿瘤 病理过程 化疗相关基因 药物筛选试验 抗肿瘤 

分 类 号：R735.2[医药卫生—肿瘤]