基于数据挖掘分析非小细胞肺癌中RPA3的表达及其临床意义  

Expression and prognostic significance of RPA3 in non-small cell lung cancer: Evidence from data mining

作　　者：王晓飞 裴永坚 汪泱[1] 苏楠[1] 雷伟[1] 曾大雄[1] 朱晔涵[1] 黄建安[1] WANG Xiaofei;PEI Yongjian;WANG Yang;SU Nan;LEI Wei;ZENG Daxiong;ZHU Yehan;HUANG Jian'an(Department of Respiratory and Critical Care Medicine,the First Affiliated Hospital of Soochow University,Suzhou Jiangsu 215006,China)

机构地区：[1]苏州大学附属第一医院呼吸与危重症医学科,江苏苏州215006

出　　处：《临床与病理杂志》2018年第11期2363-2370,共8页Journal of Clinical and Pathological Research

基　　金：国家临床重点专科建设项目;江苏省青年卫生人才项目(QNRC2016745);江苏省"科教强卫工程"青年医学人才(QNRC2016747);姑苏卫生青年拔尖人才~~

摘　　要：目的:探索复制蛋白A(replication protein A,RPA)3在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达及意义。方法:利用BioGPS数据库检索RPA3在正常组织中的表达;使用Oncomine数据库分析关于RPA3基因的信息,并对RPA3基因在肺癌中的表达进行荟萃分析;利用Kaplan-Meier Plotter数据库分析RPA3表达对NSCLC患者生存时间的影响;String-DB数据库分析与RPA3相互作用的蛋白。结果:RPA3在人体正常组织中均有表达。与正常肺组织相比,NSCLC组织中RPA3基因在mRNA水平呈高表达(P<1E-5)。RPA3基因的高表达与NSCLC患者的总体生存期(overall survival,OS)呈负相关(HR=1.48,P=2.2E-09)。亚组分析显示:RPA3高表达水平对肺腺癌患者OS有显著影响(HR=2.09,P=3.4E-09),而在鳞癌患者中,其表达水平对OS无显著影响(HR=0.97,P=0.77)。MCM2,ORC2,ORC3,PCNA,POLD1,PRIM1,RFC4,RPA1,RPA4等基因与RPA3有明显的相互作用。结论:通过肿瘤基因数据库信息挖掘表明,RPA3在NSCLC组织中呈高表达,且与患者预后相关,为深入研究RPA3在NSCLC发生发展中的作用提供重要理论依据。Objective: To explore the expression and significance of replication protein A(RPA) 3 in non-small cell lung cancer(NSCLC). Methods: Utilizing BioGPS database analyzed RPA3 expression in normal tissues. Using Oncomine database mined the expression of RPA3 in NSCLC patients. Exploiting Kaplan-Meier Plotter database studied the prognostic roles of RPA3 in NSCLC. The RPA3-interacting proteins were analyzed by String-DB database. Results: The BioGPS database shows RPA3 expression in all the human normal tissues. Compared with normal lung tissue, RPA3 gene highly expressed in NSCLC tissue at mRNA level(P<1 E-5). The expression level of RPA3 gene was negatively correlated with overall survival(OS) of NSCLC patients(HR=1.48, P=2.2 E-09). Subgroup analysis showed that RPA3 expression level had a significant effect on OS of patients with lung adenocarcinoma(HR=2.09, P=3.4 E-09), while in squamous cell carcinoma its expression level had no significant effect on OS(HR=0.97, P=0.77). The MCM2, ORC2, ORC3, PCNA, POLD1, PRIM1, RFC4, RPA1, RPA4 and other genes have obvious interaction with RPA3. Conclusion: According to the information mining of tumor gene database, RPA3 mRNA was found highly expressed in NSCLC tissue and the expression of RPA3 has a significant impact on survival of NSCLC patients, which provides an important theoretical basis for further studying the role of RPA3 in the occurrence and development of NSCLC.

关 键 词：非小细胞肺癌 复制蛋白A Oncomine 数据分析 预后 

分 类 号：R734.2[医药卫生—肿瘤]