QuEChERS/超高效液相色谱-串联质谱法测定茶叶中17种氨基甲酸酯类农药及其代谢物残留  

Simultaneous determination of 17 carbamate pesticides residues in tea by QuEChERS-UPLC-MS/MS

作　　者：张艳侠 张婷[2] 薛霞 卢兰香 杨昊 周禹君 祝建华 刘艳明 ZHANG Yan-xia;ZHANG Ting;XUE Xia;LU Lan-xiang;YANG Hao;ZHOU Yu-jun;ZHU Jian-hua;LIU Yan-ming(Shandong Institute for Food and Drug Control,Jinan 250101;Bioengineering Department,Shandong Polytechnic,Jinan 250104)

机构地区：[1]山东省食品药品检验研究院,济南250101 [2]山东职业学院生物工程系,济南250104

出　　处：《分析试验室》2019年第1期13-18,共6页Chinese Journal of Analysis Laboratory

基　　金：国家重点研发计划(2017YFC1601600)资助

摘　　要：建立了检测茶叶中灭多威等17种氨基甲酸酯类农药残留的超高效液相色谱-串联质谱(UPLC-MS/MS)方法。详细讨论了加水对茶叶中氨基甲酸酯类农药提取效率的影响。茶叶样品加水后,用乙腈提取,经QuEChERS方法净化,C18色谱柱分离,三重四极杆电喷雾质谱检测,基质外标法定量。17种氨基甲酸酯类农药残留在各自质量浓度范围内线性关系良好,相关系数(R^2)均大于0. 99。方法检出限(S/N=3)为0. 001～0. 02 mg/kg,定量限(S/N=10)为0. 002～0. 05 mg/kg。在0. 05,0. 5,5. 0 mg/kg 3个添加水平下,17种农药残留平均加标回收率在82. 1%～119. 2%之间,相对标准偏差均小于8. 5%。A method for determination of 17 carbamate pesticide residues in tea by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was established.After rehydrated,the samples were extracted with acetonitrile and purified by the Qu ECh ERS method.The separation was carried out on a C18 column by ultra performance liquid chromatography(UPLC).The target compound was detected by MS/MS system with eletrospray ionization(ESI)under a multiple reaction monitoring(MRM)mode.The calibration curves of 17 carbamate pesticides showed good linearity in their own ranges with correlation coefficients higher than 0.99.The average recoveries ranged from 82.1% to 119.2%,at three spiking levels with RSD < 8.5%.The limit of detection(LOD)and the limit of quantitation(LOQ)of carbamate pesticide residues in tea were 0.001 ~ 0.02 mg/kg and 0.002 ~ 0.05 mg/kg,respectively.

关 键 词：茶叶 氨基甲酸酯类农药 QUECHERS 超高效液相色谱-串联质谱法(UPLC-MS/MS) 

分 类 号：TS207.5[轻工技术与工程—食品科学]