白藜芦醇对非小细胞肺癌细胞自噬和凋亡的影响  

Effect of resveratrol on autophagy and apoptosis of non-small cell lung cancer cells

作　　者：张云珍 邵松军[2] 余红[2] 袁国航 方海燕 Zhang Yunzhen;Shao Songjun;Yu Hong;Yuan Guohang;Fang Haiyan(Department of Respiratory Medicine,First People's Hospital of Huizhou,Huizhou 516000,China;Department of Respiratory and Critical Medicine,Guizhou Provincial People's Hospital, Guiyang 550002,China;Department of Senile Psychiatry,the Second People's Hospital of Gnizhou Province,Guiyang 550005, China)

机构地区：[1]惠州市第一人民医院呼吸内科,惠州516000 [2]贵州省人民医院呼吸与危重症医学科,贵阳550002 [3]贵州省第二人民医院老年精神科,贵阳550005

出　　处：《中国癌症防治杂志》2018年第6期439-443,共5页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

基　　金：贵阳市科技计划资助项目(黔科合同[20161001]09号);贵州省卫生计生委科学技术基金资助项目(gzwjkj2017-1-019)

摘　　要：目的探讨白藜芦醇(resveratrol,Res)对人非小细胞肺癌A549细胞自噬和凋亡的影响。方法采用50μmol/L白藜芦醇作用于非小细胞肺癌A549细胞(Res组),对照组细胞用2%FBS的DMEM培养,培养24 h后采用倒置显微镜观察两组A549细胞形态的变化,激光扫描共聚焦显微镜观察细胞自噬小体的形成,Western blot检测自噬相关蛋白Beclin1、LC3Ⅱ/Ⅰ、p62和凋亡相关蛋白Bcl-2、Bax、活化caspase-3的表达。结果 A549细胞培养24 h后,倒置显微镜下可见Res组细胞形状较对照组变圆,细胞排列稀疏;激光扫描共聚焦显微镜下可观察到Res组A549细胞胞浆内大量自噬体形成。Western blot检测结果显示,与对照组比较,Res组A549细胞自噬相关蛋白Beclin 1、LC3Ⅱ/Ⅰ表达升高(0.151±0.032 vs 0.093±0.013,P=0.011;3.644±0.122 vs 1.903±0.054,P<0.001),p62蛋白表达下降(0.032±0.002 vs 0.061±0.005,P=0.021);促凋亡相关蛋白Bax、活化caspase-3表达升高(0.633±0.061 vs 0.423±0.053,P=0.011;0.154±0.004 vs 0.111±0.011,P=0.002),而抑制凋亡蛋白Bcl-2表达下降(2.437±0.055 vs 3.503±0.138,P<0.001)。结论白藜芦醇可通过促进非小细胞肺癌A549细胞过度自噬而诱导细胞凋亡。Objective To investigate the effects of resveratrol(Res)on autophagy and apoptosis of non-small cell lung cancer A549 cells. Methods A549 cells were divided into control group and resveratrol group(Res group). Res group cells were treated with50 μmol/L resveratrol,and the control group cells were cultured with DMEM containing 2% FBS. The morphological changes were observed by inverted microscopy. The formation of autophagosomes was observed by laser scanning confocoal. The expression of the autophagy-related proteins Beclin1,LC3Ⅱ/Ⅰ,p62 and apoptosis-related proteins Bcl-2,Bax,Active caspase-3 were detected by Western blot. Results After 24 h culture,the cell in the Res group were more round and sparsely arranged than those in the control group. A large number of autophagy bodies formed in the cytoplasm. Western blot showed that Beclin1 and and LC3 Ⅱ/Ⅰexpression were increased in A549 cells in the Res group than in the control group(0.151±0.032 vs 0.093±0.013,P=0.011;3.644±0.122 vs 1.903 ±0.054,P <0.001),but p62 protein expression was reduced(0.032 ±0.002 vs 0.061 ±0.005,P =0.021) in the Res group. The expression of pro-apoptotic protein Bax and activated caspase-3 were increased in the Res group(0.633 ±0.061 vs0.423 ±0.053,P=0.011;0.154±0.004 vs 0.111±0.011,P=0.002). However,the expression of anti-apoptotic protein Bcl-2 was reduced in the Res group(2.437±0.055 vs 3.503±0.138,P<0.001). Conclusion Resveratrol induces non-small cell lung cancer A549 cells apoptosis by promoting excessive autophagy.

关 键 词：非小细胞肺癌 A549细胞 白藜芦醇 自噬 凋亡 

分 类 号：R739.6[医药卫生—肿瘤]