黄芩素与5-氟尿嘧啶联用对肝癌耐药细胞株增殖及凋亡的诱导作用  

Effects of baicalein combined with 5-fluorouracil on proliferation and apoptosis in drug -resistant hepatocellular carcinoma cell lines

作　　者：郭瑛[1] 李君[1] 田红卫 李真真[1] 田静[1] 张栩锐 张淑群[2] 李宗芳[1] Guo Ying;Li Jun;Tian Hongwe;Li Zhenzhen;Tian Jing;Zhang Xuru;Zhang Shuqun;Li Zongfang(National & Local Joint Engineering Research Center of Biodiagnostics and Biotherapy,the Second Affiliated Hospital of Xi 'an Jiaotong University,Xi 'an 710004,China;Department of Oncology ,the Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710004,China)

机构地区：[1]西安交通大学第二附属医院生物诊断治疗国家地方联合工程研究中心,710004 [2]西安交通大学第二附属医院肿瘤科,710004

出　　处：《中华实验外科杂志》2019年第1期66-68,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金青年科学基金(81603159);中央高校基本科研业务费专项资金(XJJ2015102);西安交通大学第二附属医院科研基金青年项目(YJ(QN)201502).

摘　　要：目的观察黄芩素对肝癌耐药细胞株应用5-氟尿嘧啶(5-Fu)化疗后增殖及凋亡诱导作用的影响。方法通过培养人肝癌耐5-Fu细胞株Bel7402/5-Fu,将细胞分为对照组、5-Fu组、黄芩素组、黄芩素+5-Fu组,采用噻唑蓝实验检测细胞增殖,膜联蛋白V-异硫氰酸荧光素/碘化丙锭双染色流式细胞术实验检测细胞凋亡,明确黄芩素对耐药细胞增殖和凋亡的影响。结果黄芩素+5-Fu组与5-Fu组比较,黄芩素浓度为100 μmol/L时,能显著的增加5-Fu抑制增殖能力约为3.87倍,采用金正均Q值法评估联合用药效果发现均>1.15,两者联合用药抑制细胞活力具有协同效应;细胞总凋亡率由(7.5±0.23)%提高至(35.6±3.58)%(P<0.01),早期凋亡细胞的比例(2.2±0.06)%提高至(21.8±2.31)%(P<0.05),凋亡比例增加与黄芩素浓度呈剂量依赖性。结论 黄芩素联合5-Fu可明显抑制肝癌耐药细胞增殖,促进其凋亡,逆转细胞对5-Fu的耐药。Objective To explore the effect of baicalein on proliferation and apoptosis of drug-resistant hepatocellular carcinoma cells treated with 5-fluorouracil (5-Fu) chemotherapy. Methods Cell culture of drug-resistant hepatocellular carcinoma cell line Bel7402/5-Fu was performed. Cells were divided into control group, 5-Fu group, baicalein group, and baicalein plus 5-Fu group. The methyl thiazole tetrazolium assay was performed to detect the cell proliferation and Annexin V-fluorescein isothiocyanate/propidium iodide assay was used to investigate the apoptosis rate of the cells.Results Compared with 5-Fu alone, the viability was significantly decreased in baicalein (100 μmol/L) plus 5-Fu groups (enhanced about 3.87 times). Since Jin Zhengjun Q values were all more than 1.15, the combination efficacy was synergy. The baicalein induced a dose-dependent increase in apoptosis rate from (7.5±0.23)% to (35.6±3.58)% (P<0.01) and early apoptosis rate from (2.2±0.06)% to (21.8±2.31)% (P<0.05).Conclusion Baicalein combined with 5-fluorouracil can prominently inhibit the proliferation of drug-resistant hepatocellular carcinoma cells, promote their apoptosis, thus, reverse drug resistance to 5-Fu.

关 键 词：肝癌 黄芩素 5-氟尿嘧啶 

分 类 号：R735.7[医药卫生—肿瘤]