微小RNA-221靶向HMBOX1对胃癌细胞生物学功能影响  

Effect of microRNA -221 targeting HMBOX1 on biological function of gastric cancer cells

作　　者：马钊[1] 谷军保[1] 鲍学斌[1] Ma Zhao;Gu Junbao;Bao Xuebin(Department of Gastrointestinal Surgery,People's Hospital of Henan Province,Zhengzhou 450003,China)

机构地区：[1]河南省人民医院胃肠外科,郑州450003

出　　处：《中华实验外科杂志》2019年第1期79-82,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察微小RNA(miRNA,miR)-221p的靶点及其对胃癌细胞生物学功能的影响。方法通过生物信息学预测miR-221的可能靶点,并构建plenti-miR-221过表达载体,包装慢病毒,通过感染高转移胃癌细胞GC9811P建立miR-221稳定表达细胞株(观察组),同时建立对照细胞株(对照组)。采用Western blot检测对照组和观察组细胞miR-221靶基因蛋白表达水平,采用细胞计数试剂盒(CCK-8)检测对照组和观察组细胞增殖能力,采用Transwell检测对照组和观察组细胞侵袭能力,采用流式细胞术分析对照组和观察组细胞的凋亡水平,采用体内移植瘤实验分析对照组和观察组细胞肿瘤生长以及转移。 结果生物信息学分析显示miR-221可能的靶基因是HMBOX1。与对照组(1.12±0.23)比较,观察组细胞HMBOX1蛋白表达水平(0.32±0.12)显著下调,差异有统计学意义(t=3.976,P<0.01)。与对照组比较,观察组细胞增殖能力明显增加,差异有统计学意义(F=3.102,P<0.01)。Transwell定量分析显示观察组细胞侵袭能力[(125.43±15.32)个]显著高于对照组细胞侵袭能力[(57.40±9.81)个],差异有统计学意义(t=3.190,P<0.01)。观察组细胞凋亡水平[(12.78±3.56)%]明显低于对照组细胞凋亡水平[(25.43±7.54)%],差异有统计学意义(t=2.918,P<0.01)。观察组细胞在裸鼠体内生长速度明显高于对照组,差异有统计学意义(F=3.012,P<0.01)。观察组体内成瘤后腹膜内转移病灶数量(15.26±3.29)明显高于对照组小鼠(6.19±2.01),差异有统计学意义(t=2.991,P<0.01)。结论 miR-221可靶向作用于HMBOX1,促进肿瘤细胞增殖和侵袭,降低细胞凋亡,进而促进肿瘤发生和转移。Objective To explore the target of microRNA (miRNA, miR)-221p and its effect on biological function of gastric cancer cells.Methods Bioinformatics was used to predict the possible targets of miR-221, and plenti-miR-221 overexpression vector was constructed. Lentiviruses were packaged. MiR-221 stable expression cell line (observation group) and control cell line (control group) was established by lentiviruses infecting. The expression of miR-221 target gene protein in control and observation groups were analyzed by Western blotting. The proliferation ability in control and observation groups was detected by cell counting kit-8 (CCK-8). The invasive ability was analyzed by Transwell in control and observation groups. The apoptosis level of control and observation groups was analyzed by flow cytometry. The tumor growth and metastasis of the control group and the observation group were analyzed.Results Bioinformatics analysis showed that the target gene of miR-221 was HMBOX1. Compared with the control group (1.12±0.23), the expression of HMBOX1 protein in the observation group (0.32±0.12) was significantly decreased (t=3.976, P<0.01). Compared with the control group, the proliferation ability of cells in the observation group increased significantly (F=3.102, P<0.01). Transwell quantitative analysis showed that the invasive ability of cells in the observation group [(125.43±15.32) cells] was significantly higher than that in the control group [(57.40±9.81) cells], and the difference was statistically significant (t=3.190, P<0.01). The level of apoptosis in the observation group [(12.78±3.56)%] was significantly lower than that in the control group [(25.43±7.54)%, t=2.918, P<0.01]. The growth rate of cells in the observation group was significantly higher than that in the control group (F=3.012, P<0.01). The number of intraperitoneal metastatic lesions in the observation group (15.26±3.29) was significantly higher than that in the control group (6.19±2.01) (t=2.991, P<0.01).Conclusion MiR-221 targeting HMBOX1,

关 键 词：微小RNA-221 HMBOX1 增殖 凋亡 转移 

分 类 号：R735.2[医药卫生—肿瘤]