JNK被抑制后LPS对皮肤成纤维细胞增殖的作用机制  

Action mechanism of LPS in proliferation of skin fibroblasts after inhibition of JNK

作　　者：王永祥[1] 王春燕[1] 赵鹏伟 WANG Yong-xiang;WANG Chun-yan;ZHAO Peng-wei(Department of Laboratory Medicine,The Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010050,Inner Mongolia A utonomous Region,China)

机构地区：[1]内蒙古医科大学附属医院,内蒙古呼和浩特010050 [2]内蒙古医科大学基础医学院,内蒙古呼和浩特010049

出　　处：《中国生物制品学杂志》2019年第1期33-35,共3页Chinese Journal of Biologicals

基　　金：内蒙古自然科学基金(2017MS08116);内蒙古自治区教育厅基金支持(NJZC16115)

摘　　要：目的抑制JNK通路后,分析LPS对皮肤成纤维细胞的作用。方法用400 ng/mL JNK磷酸化激动剂Anisomycin和20 ng/mL脂多糖(lipopolysaccharide,LPS)同时作用于皮肤成纤维细胞,并设LPS单独作用组,MTT法检测作用0、24、48和72 h时的细胞生长率,Western blot法检测作用0、20、40、60 min时,细胞中ERK、JNK和P38的磷酸化情况。结果 Anisomycin和LPS同时作用皮肤成纤维细胞24 h开始,细胞生长率明显高于对应的LPS单独作用组(P <0. 05);磷酸化ERK、JNK、P38的表达未发生变化,而LPS单独作用细胞20和40 min,磷酸化JNK的表达逐渐降低(P <0. 05)。结论在LPS作用于皮肤成纤维细胞过程中,是通过JNK途径刺激皮肤成纤维细胞产生炎性反应,为进一步研究LPS作用于皮肤成纤维细胞的发病机制奠定了基础。Objective In this study,we studied the effect of Anisomycin and LPS on the growth of skin fibroblasts through MAPK pathway.Methods Skin fibroblasts were treated with 400 ng/mL Anisomycin and 20 ng/mL lipopolysaccharide(LPS),using those treated with LPS alone as control.The growth rates of fibroblasts 0,24,48 and 72 h after treatment were determined by MTT assay,while the phosphorylation of ERK,JNK and p38 0,20,40 and 60 min after treatment by Western blot.Results The growth rate of fibroblasts after treatment with Anisomycin and LPS for more than 24 h were significantly higher than that with LPS alone(P< 0.05).The expressions of phosphorylated JNK,ERK and p38 showed no changes.However,the expression level of phosphorlyated JNK in fibroblasts 20 and 40 min after treatment with LPS alone decreased gradually(P< 0.05).Conclusion The study provides an evidence that LPS inhibits the growth of skin fibroblasts mainly via JNK-dependent pathway,which lays a foundation of further study on role of LPS in pathogenic change of skin fibroblasts.

关 键 词：皮肤成纤维细胞 增殖 脂多糖 JNK通路 抑制 ANISOMYCIN 

分 类 号：Q257[生物学—细胞生物学]