胰高血糖素样肽1对2型糖尿病合并阿尔茨海默病大鼠学习记忆功能的影响  

Effect of glucagon-like peptide-1 on learning and memory abilities in Alzheimer disease and type 2 diabetes mellitus rats

作　　者：张翼鸿[1] 冯琨[1] 徐明艳[1] 肖玲[1] 杨艳[1] 王亦薇[1] 于淼[1] Zhang Yihong;Feng Kna;Xu Mingyan;Xiao Ling;Yang Yan;Wang Yiwei;Yu Miao(Department of Endocrinology,Nangang Division of Heilongjiang Provincial Hospital,Harbin 150001,China)

机构地区：[1]黑龙江省医院南岗院区内分泌科,哈尔滨150001

出　　处：《中国医药》2019年第1期126-130,共5页China Medicine

基　　金：黑龙江省青年科学基金(QC2014C114)~~

摘　　要：目的观察胰高血糖素样肽1(GLP-1)对2型糖尿病(T_2DM)合并阿尔茨海默病(AD)大鼠学习记忆功能的影响。方法 60只SD大鼠完全随机分为正常对照组、T_2DM组、AD组、T_2DM+AD组,各15只。T_2DM组、AD组和T_2DM+AD组分别制备T_2DM模型、AD模型和T_2DM+AD模型,模型制备成功后皮下注射GLP-1类似物利拉鲁肽注射液300μg/(kg·d),连用4周;正常对照组给予等体积0.9%氯化钠注射液皮下注射4周。比较干预前后大鼠体质量、空腹血糖及Morris水迷宫实验评价状况。结果干预前,与正常对照组比较,T_2DM组和T_2DM+AD组空腹血糖明显高,且T_2DM+AD组高于T_2DM组,差异均有统计学意义(均P <0. 05);干预后,T_2DM组和T_2DM+AD组空腹血糖较干预前明显下降,差异均有统计学意义(均P <0. 05)。干预前,与正常对照组比较,AD组和T_2DM+AD组逃避潜伏期明显延长[(105±12)、(114±14)s比(53±5)s],且T_2DM+AD组长于AD组,差异均有统计学意义(均P <0.05);干预后,AD组和T_2DM+AD组逃避潜伏期[(67±7)、(69±8)s]较干预前缩短,差异均有统计学意义(均P<0. 05)。干预前,与正常对照组比较,AD组和T_2DM+AD组第四象限游泳路程缩短,且T_2DM+AD组短于AD组,差异均有统计学意义(均P <0. 05);干预后,AD组和T_2DM+AD组第四象限游泳路程较干预前延长,差异均有统计学意义(均P <0. 05)。干预前,与正常对照组比较,AD组和T_2DM+AD组穿越平台次数减少,且T_2DM+AD组少于AD组,差异均有统计学意义(均P <0. 05);干预后,AD组和T_2DM+AD组穿越平台次数较干预前增多,差异均有统计学意义(均P<0.05)。结论 GLP-1对降低T_2DM鼠的血糖水平及改善AD鼠的学习记忆能力均有作用,在T_2DM合并AD的治疗领域可能有较大潜力。Objective To observe the influence of glucagon-like peptide-1(GLP-1) on learning and memory abilities in type 2 diabetes mellitus(T2DM) and Alzheimer disease(AD) rats. Methods Sixty SD rats were randomly prepared for normal control group, T2DM model group, AD model group and T2DM+AD model group, with 15 rats in each group. After modeling, the rats were given GLP-1 analogue liraglutide by subcutaneous injection at the dose of 300 μg/(kg·d) for 4 weeks;the normal control group was given isometric subcutaneous injection of 0.9% sodium chloride for 4 weeks. Body weight, fasting blood glucose level and results of Morris water maze test were analyzed. Results Before intervention, fasting blood glucose of rats in the T2DM group and T2DM+AD group were significantly higher than that in the normal control group;the level of blood glucose in the T2DM+AD group was significantly higher than that in the T2DM group(all P<0.05). After intervention, fasting blood glucose in the T2DM group and T2DM+AD group were significantly lower than those before intervention(both P<0.05). Before intervention, escape latencies in the AD group and T2DM+AD group were significantly longer than that in the normal control group[(105±12),(114±14)s vs (53±5)s];escape latencies in the T2DM+AD group was significantly longer than that in the AD group(all P<0.05). After intervention, escape latencies in the AD group and T2DM+AD group[(67±7),(69±8)s] were significantly shorter than those before intervention(both P<0.05). Before intervention, the fourth quadrant swimming distances in the AD group and T2DM+AD group were significantly shorter than that in the normal control group;the fourth quadrant swimming distance in the T2DM+AD group was significantly shorter than that in the AD group(all P<0.05). After intervention, the fourth quadrant swimming distances in the AD group and T2DM+AD group were significantly longer than those before intervention(both P<0.05). Before intervention, the numbers of platform crossings in the AD group and T2DM+AD group we

关 键 词：阿尔茨海默病 2型糖尿病 胰高血糖素样肽1 学习记忆 水迷宫实验 

分 类 号：R749.1[医药卫生—神经病学与精神病学]