‘纽荷尔’脐橙与‘尤力克’柠檬种间体细胞杂种的代谢特征分析  

Metabolic Characteristics of Interspecific Allotetraploid Somatic Hybrid Between ‘Newhall’ Navel Orange and ‘Eureka’ Lemon

作　　者：宋鑫 谭丰全 张苗 蔡元康 郭大勇[1] 解凯东[1] 伍小萌 郭文武[1] SONG Xin;TAN Fengquan;ZHANG Miao;CAI Yuankang;GUO Dayong;XIE Kaidong;WU Xiaomeng;GUO Wenwu(Key Laboratory of Horticultural Plant Biology of Ministry of Education,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学园艺植物生物学教育部重点实验室,武汉430070

出　　处：《园艺学报》2019年第1期37-46,共10页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31820103011);国家重点研发计划项目(2018YFD1000200;2017YFD0202001);教育部创新团队项目(IRT_17R45)

摘　　要：利用GC–MS(气相色谱—质谱)测定柑橘种间体细胞杂种——‘纽荷尔’脐橙(Citrus sinensis Osbeck)+‘尤力克’柠檬[Citrus limon(L.)Burm. F.]和其两个融合亲本果实的初生代谢物含量,包括可溶性糖、有机酸、氨基酸和脂肪酸;利用LC–MS(液相色谱—质谱)测定果实类黄酮含量,综合评估体细胞杂种果实的代谢特征。结果表明,体细胞杂种初生代谢物组成总体偏向‘尤力克’柠檬,苹果酸、柠檬酸和脂肪酸含量均高于两亲本,大多数氨基酸含量介于两亲本之间。体细胞杂种中共检测到48种类黄酮,整体偏向柠檬亲本;其中有33种物质含量介于两亲本之间,15种高于或低于两亲本。The levels of primary metabolite in the fruit pulp of somatic hybrid [Citrus sinensis Osbeck‘Newhall’+ Citrus limon(L.)Burm. F.‘Eureka’] and both parental species were quantitatively evaluated by GC–MS(gas chromatography-mass spectrometry)and flavonoid contents were investigated by LC–MS(Liquid chromatography-mass spectrometry). The results indicated the allotetraploid was closer to the lemon parent in the accumulation of primary metabolites. The levels of malic acid,citric acid and four fatty acids were higher than those of the diploid parents,while the contents of most amino acids in the allotetraploid showed an additive manner. Additionally,a total of 48 flavonoids were detected in the allotetraploid. The contents of 33 flavonoids were intermediate between both parents. Meanwhile,the levels of 15 flavonoids were transgressive. The allotetraploid was closer to the lemon parent in flavonoid composition.

关 键 词：柑橘 异源四倍体 体细胞杂交 初生代谢 类黄酮 

分 类 号：S666[农业科学—果树学]