海南龙血树肉桂酸-4-羟基化酶基因(DcC4H)的克隆及表达分析  

Cloning and Expression Analysis of Cinnamate 4-Hydroxylase Gene DcC4H in Dracaena cambodiana

作　　者：梁惠桢 朱家红[2] 戴好富[2] 梅文莉[2] 彭世清[2] Liang Huizhen;Zhu Jiahong;Dai Haofu;Mei Wenli;Peng Shiqing(Institute of Tropical Agriculture and Forestry,Hainan Universi.ty,Haikou,570228;Institute of Tropical Biosciences and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101)

机构地区：[1]海南大学热带农林学院,海口570228 [2]中国热带农业科学院,热带作物生物技术研究所,海口571101

出　　处：《分子植物育种》2018年第24期7984-7989,共6页Molecular Plant Breeding

基　　金：国家自然科学基金(81773845);海南省自然科学基金创新研究群体项目(2017CXTD020)共同资助.

摘　　要：肉桂酸4-羟基化酶(cinnamate 4-hydroxylase, C4H)是植物苯丙烷代谢途径中的关键酶,能够影响类黄酮次生代谢物的生物合成。本研究根据海南龙血树转录组数据,利用RT-PCR技术在海南龙血树中克隆了一个C4H基因DcC4H,该基因包含一个1 518 bp的开放阅读框,编码505个氨基酸,蛋白质分子量57.82 kD。生物信息学分析显示,DcC4H属于细胞色素氧化酶P450 Superfamily,具有Cytochrome P450保守位点;蛋白序列比对显示DcC4H与其它植物C4H同源性高,保守性强。表达结果显示,DcC4H在花中的表达显著高于根、叶、果和茎;脱落酸(ABA)、6-苄氨基腺嘌呤(6-BA)、油菜素内酯(BR)、茉莉酸(JA)、紫外线(UVB)和蔗糖处理下,DcC4H的表达量均上调,其中UVB处理表达上调最显著。本研究将为进一步研究DcC4H在海南龙血树类黄酮生物合成的功能提供理论依据。Cinnamic acid 4-hydroxylase is a key enzyme in the phenylpropane metabolic pathway. C4 H affect the biosynthesis of flavonoid secondary metabolites. Based on the transcriptional data from Dracaena cambodiana, a C4 H gene designated as DcC4 H was cloned by RT-PCR technology. DcC4 H contained a 1 518 bp open reading frame encoding 505 amino acids with a predicted molecular mass of 57.82 kD. Bioinformatics analysis showed that DcC4 H belonged to P450 superfamily and had the conserved site of cytochrome P450. DcC4 H was highly homologous to other plant’s C4 H. DcC4 H was significantly expression in flower, followed by root, leaf, fruit and stem.The transcription level of DcC4 H increased after induced by ABA, BR, 6-BA, JA, UVB and sucrose. This study will lay the foundation for further study the function of DcC4 H in Dracaena cambodiana flavonoid biosynthesis.

关 键 词：海南龙血树 类黄酮 肉桂酸-4-羟基化酶 表达分析 

分 类 号：S567.19[农业科学—中草药栽培]