大肠湿热证管状腺瘤与P53、bFGF表达的关系研究  

Study on the relationship between colorectal tubular adenoma of damp heat syndrome and the expression of P53 and bFGF

作　　者：林巧云[1] 付肖岩[2] 林建龙[3] 王薇[2] 陈小玲[2] 罗丹[2] LIN Qiao-yun;FU Xiao-yan;LIN Jian-long;WANG Wei;CHEN Xiao-ling;LUO Dan(Department of Internal Medicine,The Second People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350000,China;Department of Gastroenterology,The Second People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350000,China;Department of Pathology,The Second People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350000,China)

机构地区：[1]福建中医药大学附属第二人民医院东二环院区内科,福建福州350000 [2]福建中医药大学附属第二人民医院脾胃病科,福建福州350000 [3]福建中医药大学附属第二人民医院病理科,福建福州350000

出　　处：《中国中西医结合消化杂志》2018年第12期987-991,共5页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：福建省自然科学基金课题(No:2017J0105)

摘　　要：[目的]探讨大肠湿热证管状腺瘤与P53、bFGF表达的相关性及临床意义。[方法]选取我院确诊为大肠管状腺瘤伴上皮内瘤变(IN)患者60例,按中医辨证分为研究组(大肠湿热证组)、对照组(脾胃虚弱证组)各30例,并设立正常对照组20例(来源于本院体检中心)。通过免疫组织化学方法检测3组肠黏膜组织中P53及bFGF的表达水平,比较分析3组P53、bFGF表达情况,探讨它们之间的相关性。[结果]大肠管状腺瘤P53、bFGF的表达在性别、年龄上差异无统计学意义;在低、高级别IN程度中的表达差异有统计学意义(P<0.05),且成正相关。P53、bFGF在大肠湿热证组、脾胃虚弱证组表达均明显高于正常对照组,差异有统计学意义(P<0.05);大肠湿热证组中P53、bFGF表达与脾胃虚弱证组比较差异无统计学意义。大肠管状腺瘤伴高级别上皮内瘤变(HGIN)大肠湿热证P53表达高于脾胃虚弱证,差异有统计学意义(P<0.05);不同瘤变程度大肠湿热证组、脾胃虚弱证组中bFGF表达差异均无统计学意义(P<0.05)。大肠湿热证组和脾胃虚弱证组中P53、bFGF在HGIN中的表达均明显高于低级别上皮内瘤变(LGIN),差异有统计学意义(P<0.05)。[结论]大肠管状腺瘤HGIN中P53表达与大肠湿热证可能存在相关。P53、bFGF在大肠管状腺瘤组织中的表达明显高于正常肠黏膜组织,且HGIN中表达明显升高,提示P53及bFGF在腺瘤恶变过程中可能起重要作用。[Objective]To investigate the correlation between Colorectal tubular adenoma with damp heat syndrome of large intestine and the expression of P53 and bFGF.[Methods]Sixty cases of colorectal tubular adenoma with intraepithelial neoplasia were selected.These patients were divided into study group(group of syndrome of dampness-heat in large intestine)and control group(group of weakness of spleen and stomach)by TCM syndrome differentiation,and 30 cases in each group.And 20 cases of normal control group were established(from the hospital physical examination center).We detected the expression levels of P53 and bFGF in the intestinal mucosa of the study group,the control group and the normal control group by immunohistochemical method,then carryed out a statistical analysis to explore the correlation between them.[Results]The expression of P53 bFGF in colorectal tubular adenoma had no significant differ-ence in sex and age,but there was a significant difference in the expression of P53 bFGF in low and high grade intraepithelial neoplasia(P<0.05),and there was a positive correlation between the expression of P53 and bFGF(P<0.05).The expression of P53 and bFGF in group of syndrome of dampness-heat in large intestine and in group of weakness of spleen and stomach were significantly higher than normal control group.The difference between the group of syndrome of dampness-heat in large intestine and normal control group was statistically significant(P <0.05).And there was no significant difference compared with the group of weakness of spleen and stomach.The expression of P53 in colorectal tubular adenoma with high grade intraepithelial neoplasia was significantly higher than that in spleen and stomach asthenia syndrome(P <0.05);There was no significant difference in the expression of bFGF between the two groups(P<0.05).The expression of P53 and bFGF in group of syndrome of dampness-heat in large intestine and group of weakness of spleen and stomach was higher than that of low grade intraepithelial neoplasia(Low grade intra

关 键 词：大肠湿热证 大肠管状腺瘤 P53 BFGF 

分 类 号：R735.3[医药卫生—肿瘤]