Transcriptomics-based identification and characterization of 11 CYP450 genes of Panax ginseng responsive to MeJA  

作　　者：Xu Zeng Tiao Luo Jijia Li Gui Li Donghua Zhou Tuo Liu Xian Zou Aparna Pandey Zhiyong Luo 

机构地区：[1]Molecular Biology Research Center,School of Life Sciences,Central South University,Changsha 410006,China

出　　处：《Acta Biochimica et Biophysica Sinica》2018年第11期1094-1103,共10页生物化学与生物物理学报（英文版）

基　　金：the grants from the National Natural Science Foundation of China (Nos.81673544 and 81874332);the Fundamental Research Fund of Central South University (No. 2016zzts584);the Teacher-student Innovation and Entrepreneurship Project of Central South University (No.2017gczd028);the Natural Science Foundation of Hunan Province of China (No.2016JJ4113);the Innovation Project for University Students of Hunan Province of China (No.774);the Natural Science Fund for Young Scholars of Xiangya Hospital of Central South University (No.2014Q06).

摘　　要：Cytochromes P450 (CYP450s),a superfamily of mono-oxygenases,are essential to generate highly functionalized secondary metabolites in plants and contribute to the diversification of specialized triterpenoid biosynthesis in eudicots.However,screening and identifying the exact CYP450 genes in ginsenoside biosynthesis is extremely challenging due to existence of large quantities of members in CYP450 superfamily.Therefore,to screen the CYP450 genes involved in ginsenoside bio- synthesis,transcriptome dataset of Panax ginseng was created in our previous work using the technique of the next-generation sequencing.On the basis of bioinformatics analysis,16 putative CYP450 genes with significant differential expression were screened from the dataset and submit- ted to GenBank,in which 11 of them have been cloned.Methyl jasmonate (MeJA)was used as an elicitor to analyze the expression profiles of candidate CYP450 genes by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR).The results of qRT-PCR analysis revealed that the expression of some CYP450 genes were strongly induced by MeJA and showed different transcription levels at different treatment time points.Homology analysis indicated that each putative CYP450 protein of P.ginseng has a conserved domain consisting of E-E-R-F-P-R-G. The CYP450 genes were screened and cloned here to enrich the resources of CYP450 genes,and the results of bioinformatics analysis provided a foundation to further identify the function of CYP450s involved in ginsenoside biosynthesis.Furthermore,this study facilitated the construction of microbial cell factories for increasing the production of ginsenosides by means of metabolic engineering.

关 键 词：CYP450 family PANAX GINSENG C.A.Meyer GINSENOSIDE methyl JASMONATE expression profile phylogenetic analysis 

分 类 号：Q[生物学]