基于DNA条形码的鱼胶鉴别技术的研究  

Study of the Molecular Identification for Isinglass on DNA Barcodes

作　　者：彭继烽[1] 林晓君 郑剑红[1] 李汶[1] 王晓钰[1] 陈之敏[1] 陈一村[2] PENG Ji-feng;LIN Xiao-jun;ZHENG Jian-hong;LI Went;WANG Xiao-yu;CHEN Yi-cun PENG Ji-feng;LIN Xiao-jun(Shantou Institute for Drug Control,Shantou 515000,China;Department of Pharmacology,Shantou University Medical College,Shantou 515000,China)

机构地区：[1]广东省汕头市药品检验所,广东汕头515000 [2]汕头大学医学院药理研究室,广东汕头515000

出　　处：《汕头大学医学院学报》2018年第4期201-205,共5页Journal of Shantou University Medical College

基　　金：广东省食品药品检验检测技术创新专项(2015ZX03)

摘　　要：目的:研究建立基于DNA条形码的鱼胶种属鉴别的分子生物学方法。方法:(1)优化鱼胶DNA提取方法;(2)优化鱼胶DNA模板扩增体系;(3)通过对鱼胶COI基因进行序列分析,鉴别鱼胶的种属来源。结果:获取了3种已知新鲜品、8批鱼胶成品和2种混伪品的DNA序列。结论:初步建立了以COI基因作为DNA条形码的鱼胶分子鉴定法。Objective: To establish a stable system based on DNA barcodes for the molecular identification of isinglass. Methods: ⑴ Total DNA extraction method for isinglass has been optimized;⑵ The best PCR system was searched for DNA template of isinglass;⑶ Sequences analysis of mtDNA COI Gene was made to identify the isinglass origins. Results: mtDNA COI Gene sequences of three fresh samples and eight processed products of isinglass has been gained besides another two spurious breeds. Conclusion: A system based on DNA barcodes was preliminary established to identify isinglass and its adulterants.

关 键 词：鱼胶 COI基因 DNA条形码 

分 类 号：R932[医药卫生—生药学]