雌二醇增加脓毒症小鼠心肌电压门控钙通道α1C亚基(CACNA1C)水平  

Estradiol increases the level of myocardial voltage-gated calcium channel α1C subunit(CACNA1C) in septic mice

作　　者：冯莹[1] 方志成[1] 刘伯毅[1] 陈黎[1] 郑翔[1] FENG Ying;FANG Zhicheng;LIU Boyi;CHEN Li;ZHENG Xiang(Department of Intensive Care Unit,Taihe Hospital,Hubei University of Medicine,Shiyan 442000,China)

机构地区：[1]十堰市太和医院重症医学科,湖北十堰442000

出　　处：《细胞与分子免疫学杂志》2018年第10期914-918,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：十堰市科学技术研究与开发项目(17Y11)

摘　　要：目的探讨雌二醇对脓毒症小鼠心肌电压门控钙通道α1C亚基(CACNA1C)的影响。方法雄性昆明小鼠皮下注射苯甲酸雌二醇,在此基础上利用盲肠结扎穿刺法制作脓毒症模型。小鼠分为雌二醇处理的脓毒症组、脓毒症组、假手术组、雌二醇处理的假手术组。造模后3、6、12、24 h,实时荧光定量PCR检测左心室CACNA1C的mRNA水平,Western blot法检测CACNA1C的蛋白水平;造模后12 h,免疫组织化学染色法检测左心室CACNA1C的表达及分布情况。结果术后6 h,脓毒症组CACNA1C表达显著降低,在12 h和24 h一直处于较低水平。雌二醇处理的脓毒症组小鼠术后3 h、6 h和12 h CACNA1C的水平未见明显变化,24 h其水平显著增加。结论外源性雌二醇可提高脓毒症小鼠左心室CACNA1C水平。Objective To investigate the effect of estradiol on myocardial voltage-gated calcium channel α1 C subunit( CACNA1 C) in septic mice. Methods Estradiol benzoate was injected subcutaneously into male mice followed by sepsisinduced by cecal ligation and puncture( CLP). The experimental mice were divided into 4 groups: sepsis group,estradiol-treated sepsis group,sham group,estradiol-treated sham group. 3,6,12,24 hours after CLP,the mRNA level of CACNA1 C inleft ventricular was detected by real-time fluorescent quantitative PCR,the protein expression level of CACNA1 C wasdetected by Western blot analysis. The expression and distribution of CACNA1 C were detected by immunohistochemicalstaining at 12 hours after CLP. Results The expression of CACNA1 C was significantly decreased in sepsis group at 6 hoursand remained at this low level at 12,24 hours. The expression of CACNA1 C in estradiol-treated sepsis group did not changeat 3,6 and 12 hours,but was significantly decreased at 24 hours. Conclusion Exogenous estradiol can increase the leftventricular level of CACNA1 C in septic mice.

关 键 词：雌二醇 脓毒症 L型钙离子通道 电压门控钙通道α1C亚基(CACNA1C) 

分 类 号：R363.2[医药卫生—病理学] R392-33[医药卫生—基础医学]