2007—2016年四川省德尔卑沙门菌耐药与分子分型分析  

Characteristics of drug resistance and molecular typing for Salmonella Derby isolated in Sichuan Province, 2007-2016

作　　者：吕虹[1] 雷高鹏[1] 黄伟峰[1] 黄玉兰[1] 杨小蓉[1] LYU Hong;LEI Gaopeng;HUANG Weifeng;HUANG Yulan;YANG Xiaorong(Sichuan Center for Disease Control and Prevention,Sichuan Chengdu 610041,China)

机构地区：[1]四川省疾病预防控制中心,四川成都610041

出　　处：《中国食品卫生杂志》2018年第6期570-576,共7页Chinese Journal of Food Hygiene

基　　金：四川省医学会课题(S15070)

摘　　要：目的研究2007—2016年四川省德尔卑沙门菌分子分型和耐药趋势,掌握四川省德尔卑沙门菌污染状况,为暴发预警、溯源调查及抗生素使用策略提供参考数据。方法运用脉冲场凝胶电泳(PFGE)和微量肉汤稀释法对2007—2016年四川省自临床病例、食品从业人员、食物中毒样品、养殖动物及零售食品中分离的106株德尔卑沙门菌进行分子分型及14种抗生素的敏感性测试。结果 106株德尔卑沙门菌对8类14种抗生素均有不同程度耐药,人源性和动物源性菌株对四环素、萘啶酸、氯霉素、复方磺胺的耐药率均大于20%。其中人源性菌株对头孢噻肟、环丙沙星、氨苄西林、氨苄西林/舒巴坦、头孢唑啉的耐药率均明显高于动物源性菌株;动物源性菌株对四环素、萘啶酸、氯霉素耐药率均明显高于人源性菌株。经Xba I酶切后106株菌共分为67个PFGE带型,不同年份的临床病例、食品从业人员及零售食品生猪肉分离株具有相同PFGE带型。结论四川省德尔卑沙门菌分离株耐药率较高,并有逐年上升趋势。PFGE型别呈多样性,部分临床病例、食品从业人员分离株与生猪肉分离株PFGE具有相同带型。Objective To summarize and analyze the molecular typing and drug resistance trends of Salmonella Derby(S.Derby)in Sichuan Province during the past 10 years from 2007 to 2016,so as to understand the contamination status of S.Derby in Sichuan,and provide reference data for outbreak warning,traceability investigation and antibiotic use strategy.Methods Using pulse field gel electrophoresis(PFGE)and micro broth dilution to test the molecular typing and susceptibility of 14 antibiotics for 106 strains of S.Derby from Sichuan Province from 2007 to 2016,which were isolated from clinical patients,food practitioners,food poisoning outbreaks,breeding animal and retail food.Results Result showed that 106 strains of S.Derby had different levels of resistance to 14 kinds of antibiotics.The resistance rates of human and animal source strains to tetracycline,nalidixic acid,chloramphenicol and compound sulfonamide were all above 20%.The resistance rates of human source strains to cefotaxime,ciprofloxacin,ampicillin,ampicillin/sulbactam and cefazolin were higher than those of animal source.The resistance rates of animal source strains to tetracycline,nalidixic acid and chloramphenicol was higher than that of human source.A total of 106 strains of S.Derby were divided into 67 PFGE patterns by digestion with Xba I.The clinical patients food practitioners and retail raw pork isolates from different years had the same PFGE pattern.Conclusion The situation of drug resistance of S.Derby isolates in Sichuan was rather serious with an increasing trend.PFGE types were diverse.The clinical patients food practitioners and retail raw pork isolates from different years had the same PFGE pattern.

关 键 词：德尔卑沙门菌 分子分型 脉冲场凝胶电泳 耐药 四川 

分 类 号：R155[医药卫生—营养与食品卫生学]