钛合金种植体高压阳极氧化载铜/锌膜层的动物实验研究  

Experimental animal study on high voltage anodic oxidation copper/zinc film layer of titanium alloy implants

作　　者：张永佳 刘继光[1] 张慧明[1] 李慕勤[1] 姚海涛[1] 李艳芳 吴姗姗 ZHANG Yongjia;LIU jiguang;ZHANG Huiming;LI Muqin;YA0 Haitao;LI Yanfang;WU Shanshan(Key Laboratory of College Biomedical Materials of Heilongjiang Province Colleges, Jiamusi University,Jiamusi Heilongjiang 154007,China)

机构地区：[1]佳木斯大学黑龙江省高校生物医学材料重点实验室,黑龙江佳木斯154007

出　　处：《中国体视学与图像分析》2018年第4期386-394,共9页Chinese Journal of Stereology and Image Analysis

基　　金：国家自然科学基金项目(No.31370979)

摘　　要：目的研究钛合金种植体表面载铜/锌对骨结合的影响。方法酸蚀为A组,酸蚀-高压阳极氧化为B组,酸蚀-高压阳极氧化-载铜为C组,酸蚀-高压阳极氧化-载铜/锌为D组,通过大体观察,免疫荧光标记技术,锥形束投照计算机重组断层影像设备(Cone beam CT,CBCT),扫描电镜(SEM)和能谱(EDS)等检测探讨种植体与骨结合情况。结果免疫荧光技术表明8周荧光条带宽大且优于2周、4周。组内比较F=37. 119,P<0. 001,组间比较F=21. 230,P<0. 001,有统计学意义。CBCT显示8周种植体周围阴影面积最小且4周小于2周。骨界面Ca、P元素分布D组更为活跃,优于C组,B组优于A组。结论钛合金种植体经酸蚀高压阳极氧化后加载铜/锌可以增强表面活性从而促进骨结合。Objective To study the effect of copper/zinc on bone binding on the surface of titaniumalloy implants. Methods Acid etching was group A,acid etching-high voltage anodizing was group B,acid etching-high voltage anodic oxidation-with a copper film layer was group C,acid etching-high voltage anodic oxidation-with a copper/zinc layer was group D. The implant-bone binding is investigated by general observation,fluorescence labeling technique,cone beam computed tomography(cone beam CT,CBCT),scanning electron microscopy(SEM) and energy spectroscopy(EDS),Results Immunofluorescence technique showed that the fluorescent strips of 8 weeks had a larger bandwidth and was better than those of 2 weeks and 4 weeks. Inner-group comparison shows F = 37.119,P<0.001,and intra group comparison shows F = 21. 230,P < 0. 001,i. e.,with statistical significance.CBCT showed that the shadow area around the implant is the smallest for 8 weeks,and it is less for4 weeks than for 2 weeks. The distribution of Ca and P elements in the bone interface was most active in group D,and less in the order of group C,group B,and group A. Conclusions The loading of copper/zinc after high-voltage anodic oxidation of titanium alloy implants can enhance surface activity and promote bone bonding.

关 键 词：钛合金种植体 高压阳极氧化 铜 锌 骨结合 

分 类 号：TB42[一般工业技术] R-33[医药卫生]