IL-33在脑缺血再灌注小鼠脑内的表达和细胞定位  

The expression and cellular localization of IL-33 in the brain of mice with cerebral ischemia-reperfusion

作　　者：赵文彦 钟佳雯 观佩君 冯慧瑶 刘欣琪 邓文君 刘艺琳 涂幸 罗利 Zhao Wenyan;Zhong Jiawen;Guan Peijun;Feng Huiyao;Liu Xinqi;Deng Wenjun;Liu Yilin;Tu Xing;Luo Li(Guangdong Pharmaceutical University,Undergraduate,Class of 2017,School of Clinical Medicine,Guangzhou 510006;Guangdong Pharmaceutical University,Undergraduate Class of 2014,School of Bioscienees&Biopharmaeeuties,Guangzhou 510006;Guangdong Pharmaceutical University,Undergraduate,Class of 2016,School of Public Health,Guangzhou 510006;Guangdong Pharmaceutical University,Department of Anatomy and Histology and Embryology,School of Basic Medicine,Guangzhou 510006)

机构地区：[1]广东药科大学临床医学院,2017级广州510006 [2]广东药科大学生命科学院与制药学院,2014级广州510006 [3]广东药科大学公共卫生学院,2016级广州510006 [4]广东药科大学基础学院人体解剖学与组织胚胎学系,广州510006

出　　处：《神经解剖学杂志》2019年第1期52-56,共5页Chinese Journal of Neuroanatomy

基　　金：广东药科大学"创新强校工程"资助项目;广东药科大学2017年度大学生创新创业训练计划项目;广东省2017年度大学生创新创业训练计划项目;广东省自然科学基金(2018A030313579)

摘　　要：目的:通过建立脑缺血再灌注小鼠模型,观察白细胞介素-33(IL-33)阳性细胞在脑缺血再灌注小鼠大脑中的表达和定位情况,初步探讨IL-33在缺血性脑卒中中的作用。方法:采用线栓法制作右侧大脑中动脉阻塞(MCAO)建立脑缺血再灌注小鼠模型,利用TTC染色检测MCAO后脑梗死形成情况,采用免疫荧光组织化学染色法检测IL-33在脑缺血再灌注小鼠大脑中的表达。结果:成功建立脑缺血再灌注小鼠模型,脑缺血再灌注可引起小鼠大脑内IL-33表达升高;脑缺血再灌注小鼠模型脑内广泛表达IL-33。IL-33在神经元、星形胶质细胞、小胶质细胞内均有表达。结论:脑缺血再灌注小鼠脑内IL-33表达增加,IL-33可能参与小鼠缺氧缺血性脑损伤过程。Objective: To establish cerebral ischemia-reperfusion mouse model and detect interleukin-33( IL-33)expression inbrain. Methods: The cerebral ischemia reperfusion mouse model( MCAO) was established by using the thread embolization method,the cerebral infarction formation after MCAO was detected by TTC staining,and the expression of IL-33 in the brain of cerebral ischemia reperfusion mice was detected by immunofluorescence histochemical staining. Results: The mouse model of cerebral ischemia reperfusion was established successfully. The expression of IL-33 in brain could be increased. Il-33 widely expression in brain of mouse model of cerebral ischemia reperfusion.IL-33 is expressed in neurons,astrocytes,and microglia. Conclusion: The expression of IL-33 increased in brain of cerebral ischemia reperfusion mice and IL-33 may be involved in hypoxic ischemic brain injury in mice.

关 键 词：脑缺血再灌注 白细胞介素-33 免疫荧光组织化学 小鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]