红花籽粕不同极性部位体外抗氧化活性研究  

In vitro antioxidant activities in different polarities parts extract from the Safflower meal

作　　者：田华[1,2] 信学雷[2] 郭芳 阿吉艾克拜尔.艾萨 TIAN Hua;XIN Xue-lei;GUO Fang;Ajiaikebaier·Aisa(Central Laboratory,Xinjiang Military Region Center for Disease Control and Prevention,Urumqi Xinjiang,830011,China;Xinjiang Institute of Physical and Chemical Technology,Chinese Academy of Sciences,Urumqi Xinjiang,830011,China;Department of Obstetrics and Gynecology,General Hospital of Xinjiang Military Region,Urumqi Xinjiang,830011,China)

机构地区：[1]新疆军区疾病预防控制中心中心实验室,新疆乌鲁木齐830011 [2]中国科学院新疆理化技术研究所,新疆乌鲁木齐830011 [3]新疆军区总医院妇产科,新疆乌鲁木齐830011

出　　处：《职业与健康》2019年第2期185-188,192,共5页Occupation and Health

基　　金：国家自然科学基金新疆联合基金(U1203203);国家科技支撑计划项目(2011DA105B05)

摘　　要：目的测定红花籽粕不同极性提取物的抗氧化活性。方法利用不同极性的溶剂提取并萃取出红花籽粕的24个萃取物,并采用1,1-二苯基-2-三硝基苯肼(DPPH)法、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)法和对硝基酚邻酸盐(PNPP)法对抗氧化能力进行测定。结果 95%乙醇提取物氯仿萃取物提取率最高。不同极性提取物对DPPH和ABTS自由基均有一定的清除作用,对蛋白酪氨酸磷酸1B酶(PTP1B)活性有抑制作用。结论红花籽粕具有较好的抗氧化能力,具有开发利用的价值。[Objective] To determine the antioxidant activities of different polarities parts extract from the Safflower meal.[Methods] 24 extracts of Safflower meal were obtained in different fractions by using different polarities of solvents. The antioxidant effect on each substance was detected by the method of DPPH,ABTS and PNPP. [Results] 95% ethyl alcohol of trichloromethane fraction has the highest rating. The extracts from the Safflower meal had strong radical scavenging activity towards DPPH radical and ABTS radical, and had inhibitory effect on protein tyrosine phosphatase 1 B(PTP1 B)activity.[Conclusion] The extracts in different parts of Safflower meal have antioxidant activity, which provides references for development and utilization of Safflower meal.

关 键 词：红花籽粕 抗氧化性活性 自由基 

分 类 号：R115[医药卫生—公共卫生与预防医学]