固相萃取-液相色谱-串联质谱法检测番茄酱中6种交链孢霉毒素  

Determination of 6 kinds of Alternaria toxins in ketchup by solid phase extraction-liquid chromatography-tandem mass spectrometry

作　　者：吴振兴 程果 肖西志 静平 许艳丽 赵华梅 WU Zhen-Xlng;CHENG Guo;XIAO Xi-Zhi;JING Ping;XU Yan-Li;ZHAO Hua-Mei(Qingdao Customs,Qingdao 266000,China;Dagang Customs,Qingdao 266000,China)

机构地区：[1]青岛海关,青岛266000 [2]大港海关,青岛266000

出　　处：《食品安全质量检测学报》2019年第3期700-704,共5页Journal of Food Safety and Quality

基　　金：原山东出入境检验检疫局科研项目(SK201701)~~

摘　　要：目的建立固相萃取-液相色谱-串联质谱法检测番茄酱中6种交链孢霉毒素的分析方法。方法番茄酱样品经提取液提取,固相萃取柱净化后,采用液相色谱-串联质谱测定交链孢霉毒素,外标法定量。结果番茄酱样品中交链孢霉毒素添加浓度在2.0～200.0μg/kg时,回收率在66.5%～113.5%之间。检测低限为2.0～50.0μg/kg,相对标准偏差(relative standard deviation, RSD)在2.5%～9.8%。结论该方法简单快速、灵敏度高、定量准确,适合用于番茄酱中6种交链孢霉毒素的检测。Objective To establish a method for determination of 6 kinds of Alternaria toxins in ketchup by solid phase extraction-liquid chromatography-tandem mass spectrometry. Methods The ketchup sample was extracted by extracting solution, purified by solid phase extraction column, and Alternaria toxins was detected by liquid chromatography-tandem mass spectrometry, and quantified by external standard method. Results When the concentration of the Alternaria toxin in the tomato ketchup sample was 2.0-200.0 μg/kg, the recovery rates were 66.5%-113.5%. The detection low limit was 2.0-50.0 μg/kg, and the relative standard deviation(RSD) was 2.5%-9.8%. Conclusion This method is simple, rapid, sensitive and accurate, which is suitable for the determination of 6 kinds of Alternaria in tomato sauce.

关 键 词：交链孢霉毒素 固相萃取净化 液相色谱-串联质谱法 

分 类 号：O657.63[理学—分析化学] TS255.7[理学—化学]