TGFβ/Smad信号通路在口腔鳞癌热疗中的表达变化  

Changes of TGFβ/Smad signaling expression in oral squamous cell carcinoma after hyperthermia

作　　者：周昊[1] 刘奕[1] 廖楚航[1] 郭骏[1] 费伟[1] ZHOU Hao;LIU Yi;LIAO Chuhang;GUO Jun;FEI Wei(Department of Stomatology,Sichuan Academy of Medical Sciences &Sichuan Provincial People's Hospital,China,Chengdu610072)

机构地区：[1]四川省医学科学院.四川省人民医院口腔科,成都610072

出　　处：《实用口腔医学杂志》2019年第1期24-27,共4页Journal of Practical Stomatology

基　　金：自然科学基金青年基金(编号:81600852)

摘　　要：目的:探讨TGFβ/Smad信号通路在口腔鳞状细胞癌(OSCC)热疗之后相关分子的表达变化。方法:利用实时荧光定量RT-PCR和Western blot检测局部加热对OSCC荷瘤裸鼠肿瘤细胞中TGFβ,Smad2,Smad3,Smad4和Smad7的mRNA和蛋白表达的变化。结果:加热组(n=10)中TGFβ、Smad2、Smad3的mRNA表达比未加热组(n=10)明显降低(P <0. 05),Smad7的mRNA表达则明显升高(P <0. 05),而Smad4的mRNA表达无明显变化; TGFβ、Smad7、Smad4蛋白的表达变化与mRNA一致,而Smad2和Smad3的蛋白表达无明显变化,磷酸化的Smad2和Smad3(p Smad2和p Smad3)蛋白表达则明显降低(P <0. 05)。结论:TGFβ/Smad信号系统在热疗抗肿瘤效应中起着重要作用,Smad7对R-Smad磷酸化的抑制可能是其关键。Objective: To study the changes of TGFβ/Smad signaling expression in oral squamous cell carcinoma( OSCC) after hyperthermia. Methods: The expression of TGFβ,Smad2,3,4 and 7 in OSCC of tumor bearing nude mice were examined by quantitative real-time RT-PCR and Western blot respectively. Results: The mRNA expression of TGFβ,Smad2 and 3 was decreased significantly,and the mRNA levels of Smad7 was elevated in hyperthermia( HT) group( n = 10)( P < 0. 05),but the mRNA expression of Smad4 did not change significantly compared with that of control group( n = 10). The changes of protein expression of TGFβ,Smad7 and 4 were consistent with that of mRNA,the expression of Smad2 and 3 was not significantly different between the groups,but the expression of p Smad2 and p Smad3 decreased dramatically in HT group( P < 0. 05). Conclusion: TGFβ/Smad signaling exhibits important role in the antitumor effects of hyperthermia,and the inhibition of Smad7 on R-Smad phosphorylation may play a key role.

关 键 词：热疗 口腔鳞状细胞癌(OSCC) TGFβ/Smad信号通路 

分 类 号：R739.8[医药卫生—肿瘤]