口腔癌患者循环外泌体促进舌癌细胞CAL27增殖、迁移、侵袭中的作用  

The effects of circulating exosomes derived from oral cancer patients on the proliferation,migration and invasiveness of tongue cancer CAL27 cells

作　　者：郭怡[1] 彭红[1] 蹇淑娟 陈慧[1] 代喆颖 江玲 李秀臣 崔晓燕[1] GUO Yi;PENG Hong;JIAN Shujuan;CHEN Hui;DAI Zheying;JIANG Ling;LI Xiuchen;CUI Xiaoyan(Tongren Hospital of Wuhan University,Wuhan Third Hospital,China,430000)

机构地区：[1]武汉市第三医院.武汉大学附属同仁医院口腔科,430000

出　　处：《实用口腔医学杂志》2019年第1期60-65,共6页Journal of Practical Stomatology

基　　金：武汉市卫生和计划生育委员会科研项目(编号:WZ16D14)

摘　　要：目的:探讨口腔癌患者外周血中循环外泌体(CEXO)对口腔癌细胞增殖、侵袭中作用。方法:纳入口腔鳞状细胞癌患者20例。梯度离心法收集口腔癌患者外周血中循环外泌体,以透射电镜和蛋白印迹法鉴定收集所得的沉淀。将收集的外泌体加到舌癌细胞CAL27的培养中,MTT法检测细胞增殖能力,划痕愈合试验检测细胞迁移能力,Transwell小室法检测细胞侵袭能力,最后利用CAL27细胞裸鼠移植瘤模型,检测口腔癌患者循环外泌体在体内对癌细胞增殖能力的影响。结果:在体外实验中,口腔癌患者循环外泌体在体外可促进舌癌细胞增殖、迁移、侵袭;在裸鼠移植瘤模型中亦可促进口腔癌细胞增殖。结论:口腔癌患者外周血循环外泌体可促进口腔癌细胞的增殖、迁移和侵袭。Objective: To investigate the effects of circulating exosomes derived from oral cancer patients on the proliferation,migration and invasion of tongue cancer CAL27 cells. Methods: Circulating exosomes were isolated from the peripheral blood of 20 oral cancer patients using gradient centrifugation. The characteristics of the exosome was studied by transmission electron microscope( TEM) and Western blot. After treatment with circulating exosomes,CAL27 cell proliferation was evaluated with MTT assay,migration was observed by cell wound scratch assay and the invasion of CAL27 cells was tested by Transwell assay. CAL27 cell bearing nude mice were used to estimate the effect of circulating exosome on CAL27 cell proliferation and metastasis in vivo. Results: The proliferation,migration and invasion of CAL27 cells were enhanced by circulating exosomes from oral cancer patients. In vivo study showed that the tumorigenesis and metastasis of CAL27 cells were enhanced by circulating exosomes. Conclusion: Circulating exosomes from oral cancer patients can promote the proliferation,migration,invasion and metastasis of CAL27 cells.

关 键 词：口腔癌 循环外泌体(CEXO) 细胞增殖 肿瘤侵袭 

分 类 号：R739.8[医药卫生—肿瘤]