STAT3基因在急性髓系白血病中的表达及临床意义  

Expression and Clinical Significance of STAT3 Genes in Patients with Acute Myeloid Leukemia

作　　者：黄成双 谭梅[1] 张相梅 罗茜[1] 田润梅[1] 苏琼 吴柳松[1] 陈艳[1] HUANG Cheng-Shuang;TAN Mei;ZHANG Xiang-Mei;LUO Xi;TIAN Run-Mei;SU Qiong;WU Liu-Song;CHEN Yan(The Affiliated Hospital of Zunyi Medical College/The Children's Hospital of Guizhou Province,Zunyi 563003,Guizhou Province,China)

机构地区：[1]遵义医学院附属医院贵州省儿童医院,贵州遵义563003

出　　处：《中国实验血液学杂志》2019年第1期45-51,共7页Journal of Experimental Hematology

基　　金：国家自然科学基金(81370612);国家自然科学基金应急管理项目(81641008);遵义医学院2017年度学术新苗培养及创新探索专项(CK-1130-004)

摘　　要：目的:探讨STAT3基因在初诊急性髓系白血病(AML)患者骨髓中的表达及其与临床特征的相关性。方法:采用实时荧光定量PCR检测38例AML患者骨髓中STAT3 mRNA表达水平,分析其与临床特征及预后的相关性;利用Western blot检测AML患者骨髓中STAT3蛋白表达水平。选择15例正常人作为对照组。结果:在mRNA水平上,AML组的STAT3表达水平明显高于正常对照组(P<0.05);AML组的STAT3表达水平与危险程度呈正相关(P=0.01,r=0.592),高危组的STAT3表达水平明显高于标危组及对照组,其差异均有统计学意义(P<0.01,P<0.01),其余各组间差异无统计学意义(P> 0.05);M5组STAT3表达水平显著高于对照组,且有统计学差异(P=0.01);M2与M5及对照组之间STAT3表达水平无统计学差异(P=1,P=0.066)。STAT3低表达组患者的中位生存时间长于高表达组,但其差异无统计学意义(P=0.146)。在蛋白水平上,AML组STAT3表达水平高于对照组(P=0.017)。结论:STAT3基因在AML患者中存在高表达,且与患者免疫分型、危险度具有相关性。Objective: To investigate the expression of STAT3 gene in patients with acute myeloid leukemia and its correlation with clinical characteristics Methods: The real-time quantitative RT-PCR was used to detect the level of STAT3 mRNA in bone marrow samples from 38 newly diagnosed patients with acute myeloid leukemia(AML), and its relevance with clinical characteristics and prognosis were statistically analyzed. Western blot was employed to detect the STAT3 protein level in AML patients. The bone marrow cells from 15 healthy subjects were used as control. Results: At the mRNA level, the expression level of STAT3 in the AML group was significantly higher than that in control group(P<0.05). The level of STAT3 in AML group correlated positively with the risk factors of patients(P<0.01,r=0.592). The STAT3 expression level in the high-risk group was statistically higher than that in the standard-risk group and the control group(P<0.01,P<0.01). Furthermor, there was no statistical difference between the sub-groups of AML(P>0.05). The median survival time of patients in STAT3 low expression group was logner than that in high expression group, but the difference was not statistically significant(P>0.005). The level of STAT3 protein in AML patients was significantly higher than that in control group(P<0.05). Conclusion: The STAT3 gene is highly expressed in AML patients, which may be used as a predictor for high-risk of AML.

关 键 词：STAT3基因 急性髓系白血病 表达水平 临床意义 

分 类 号：R733.71[医药卫生—肿瘤]