弥漫大B细胞淋巴瘤患者外周血CD14^+单核细胞TF、VEGF表达的临床意义  

Clinical Significance of TF and VEGF Expressions on Peripheral CD14 Positive Monocytes in Patients with Diffuse Large B Cell Lymphoma

作　　者：江亚军[1] 朱贵华[1] 何耀[1] 柴星星[1] 杨小云[1] 孟凡静 庄万传[1] JIANG Ya-Jun;ZHU Gui-Hua;HE Yao;CHAI Xing-Xing;YANG Xiao-Yun;MENG Fan-Jing;ZHUANG Wan-Chuan(Department of Hematology,The Second People's Hospital of Lianyungang,Lianyungang 222006,Jiangsu Province,China)

机构地区：[1]连云港市第二人民医院血液科

出　　处：《中国实验血液学杂志》2019年第1期67-73,共7页Journal of Experimental Hematology

基　　金：连云港市医学创新团队项目;连云港市"521工程"科研项目;连云港市"科教强卫工程"青年科技项目(QN1605)

摘　　要：目的:探讨弥漫大B细胞淋巴瘤(DLBCL)患者外周血CD14^+单核细胞组织因子(TF)和血管内皮生长因子(VEGF)表达的临床意义。方法:应用流式细胞术检测41例DLBCL患者(病例组)化疗前、4个疗程化疗后以及20例健康体检者(对照组)外周血CD14^+单核细胞TF和VEGF的表达水平,分析TF和VEGF表达与国际预后指数(IPI)、短期疗效以及生存的关系。结果:病例组外周血CD14^+单核细胞TF、VEGF的表达水平显著高于对照组(P <0.01),2者表达呈正相关(r=0.755,P <0.01)。不同性别、年龄、亚型病例组CD14^+单核细胞TF、VEGF表达水平均无明显差异(P> 0.05)。具有预后危险因素患者CD14^+单核细胞TF、VEGF表达水平与不具有预后危险因素者比较均明显增高(P <0.05)。随着IPI指数的增高,病例组CD14^+单核细胞TF和VEGF表达均有递增趋势(P <0.01)。缓解组化疗前CD14^+单核细胞TF、VEGF表达水平低于未缓解组(P均<0.01);化疗后缓解组CD14^+单核细胞TF、VEGF表达水平均低于化疗前水平(P <0.01),未缓解组TF和VEGF表达水平较化疗前变化不大(P> 0.05)。TF和VEGF低表达组生存状况均优于TF、VEGF高表达组(P <0.05)。结论:DLBCL患者外周血CD14^+单核细胞高表达TF和VEGF,与国际预后指数、疗效、生存相关,对评估疗效、预后具有参考意义。Objective: To investigate the clinical significance of tissue factor(TF) and vascular endothelial growth factor(VEGF) expression on peripheral blood CD14 positive monocytes in patients with diffuse large B cell lymphoma(DLBCL). Methods: The expressions of TF and VEGF on peripheral CD14^+ monocytes in 41 patients with DLBCL(DLBCL group) before chemotherapy and after 4 chemotherapeutic courses, and in 20 healthy subjects(control group) were detected by flow cytometry respectively, meanwhile, the relationship of the expression of TF and VEGF with international prognostic indexes(IPI) and short-term effects were analysed. Results: The expression levels of TF and VEGF on peripheral CD14^+ monocytes in DLBCL group were significantly higher than those in control group(P<0.01), and a positive correlation was found between the two groups(r=0.755, P<0.01). The expression of TF and VEGF on CD14^+ monocytes in patients with prognostic risk factors significantly increased as compared with those in patients without prognostic risk factors(P<0.05), but there were no significant differences of TF and VEGF expressions on CD14^+ monocytes in DLBCL group with different sex, age, subtypes(P>0.05). As compared with patients without prognostic risk factors, the expression levels of TF and VEGF on CD14^+ monocytes of patients with prognostic risk factors significantly increased(P<0.05). The expression of TF and VEGF on CD14^+ monocytes in DLBCL group showed an increasing tendency along with the increase of IPI index(P<0.01). The expression levels of TF and VEGF on CD14^+ monocytes in remission group before chemotherapy were lower than those in non-remission group(P<0.01);after chemotherapy, the expression levels of TF and VEGF on CD14^+ monocytes in remission group were lower than those before chemotherapy(P<0.05), while the TF and VEGF expression levels in non-remission group were no singnificauly different from TF and VEGF levels before chemtherapy(P>0.05), the survival of patients in group with low expression of TF and VEGF was supe

关 键 词：弥漫大B细胞淋巴瘤 组织因子 血管内皮生长因子 国际预后指数 单核细胞 

分 类 号：R733.1[医药卫生—肿瘤]