C/EBPα基因在老年多发性骨髓瘤中的表达及临床意义  

Expre ssion and Clinical Significance of C/EBPα Gene in Elderly Multiple Myeloma

作　　者：臧玉柱[1] 陈香丽[1] 白炎亮[1] 张文荟[1] 于润红 ZANG Yu-Zhu;CHEN Xiang-Li;BAI Yan-Liang;ZHANG Wen-Hui;YU Run-Hong(Department of Hematology,Henan Province People's Hospital,Zhengzhou 450003,Henan Province,China)

机构地区：[1]河南省人民医院血液科,河南郑州450003

出　　处：《中国实验血液学杂志》2019年第1期123-128,共6页Journal of Experimental Hematology

摘　　要：目的:研究C/EBPα基因在老年多发性骨髓瘤(MM)中的表达变化及其临床预后意义。方法:自2015年2月-2017年10月在我院接受治疗的69例老年MM患者作为MM组,同时选取38例健康体检者作为对照组,采集2组人员骨髓并分离单个核细胞。采用RT-PCR方法检测样本中单个核细胞的C/EBPα基因表达水平,采用Western blot方法检测C/EBPα蛋白表达水平,采用免疫组织化学方法检测骨髓组织中的C/EBPα水平,探讨C/EBPα基因表达与MM患者临床特征及生存期的相关性。结果:MM患者中C/EBPα基因mRNA及其蛋白表达水平均明显低于对照组(P <0. 05)。C/EBPα表达水平与MM患者的ISS分期、CRP、Ca^(2+)、β2-MG、LDH及骨髓瘤细胞比例均有明显相关性(P <0. 05)。C/EBPα基因的表达与MM患者的性别、年龄、免疫球蛋白分型、Hb水平无相关性(P>0. 05)。通过免疫组织化学实验发现,对照组骨髓样本染色较深,CR、PR及复发的MM患者骨髓样本染色依次减轻。通过Western blot检测发现,CR组MM患者C/EBPα蛋白的水平均显著高于PR和复发组患者(P <0. 05)。KaplanMeier生存分析发现,C/EBPα基因高表达的患者组OS和DFS均优于低表达组(P <0. 05)。多因素Cox回归分析表明,CRP、骨髓瘤细胞比例及C/EBPα基因表达水平是影响OS及PFS独立因素(P <0. 05)。结论:C/EBPα基因在MM患者中表达水平较低,其低水平表达可能刺激MM的发生; C/EBPα基因表达与MM疾病发展密切相关。Objective: To investigate the expression of C/EBPα gene in elderly patients with multiple myeloma( MM)and its prognostic significance. Methods: Sixty-nine olderly patients with multiple myeloma( MM) treated in our hospital from February 2015 to October 2017 were selected and enrolled in the MM group,38 healthy persons received physical examination were selected and enrolled in the control group. The bone marrow of 2 groups was collected and the mononuclear cells were isolated. The mRNA expression level of C/EBPα gene in mononuclear cells was determined by RT-PCR,the Western blot was used to detect the protin expression level of PBMNC C/EBPα,and the protein level of C/EBPα in bone marrow was detected by immunohistochemistry. The correlations of C/EBPα gene expression with the clinical characteristics and survival time in MM patients were analyzed. Results: The expression level of mRNA and protein of C/EBPα in MM patients was significantly lower than that in the control group( P < 0. 05). The expression level of C/EBPα gene significantly correlated with the ISS stage,CRP,Calcium,β2-MG,LDH and the percentage of myeloma cells in MM patients( P < 0. 05). The expression of C/EBPα gene was not correlate with sex,age,immunoglobulin typing,Hb in MM patients( P > 0. 05). Immunohistochemical staining showed that the bone marrow samples of the control group were stained more deeply,and the staining intensity in bone marrow samples of MM patients with CR,PR and relapse was successively descended. The protein level of C/EBPα in CR patients with MM was significantly higher than that in PR and relapsed patients by Western blot( P < 0. 05). Kaplan-Meier survival analysis showed that OS and DFS in the patients with high expression of C/EBPα gene were higher than those in low expression group( P < 0. 05).Multivariate Cox regression analysis showed that CRP,ratio of myeloma cells and C/EBPα gene were independent factors affecting OS and PFS( P < 0. 05). Conclusion: The expression level of C/EBPα gene in MM patients is l

关 键 词：C/EBPα基因 多发性骨髓瘤 临床预后 

分 类 号：R733.3[医药卫生—肿瘤]