Whole-Genome Resequencing of a Worldwide Collection of Rapeseed Accessions Reveals the Genetic Basis of Ecotype Divergence  

作　　者：Dezhi Wu Zhe Liang Tao Yan Ying Xu Lijie Xuan Juan Tang Gang Zhou Ulrike Lohwasser Shuijin Hua Haoyi Wang Xiaoyang Chen Qian Wang Le Zhu Antony Maodzeka Nazim Hussain Zhilan Li Xuming Li Imran Haider Shamsi Ghulam Jilani Linde Wu Hongkun Zheng Guoping Zhang Boulos Chalhoub Lisha Shen Hao Yu Lixi Jiang 

机构地区：[1]institute of Crop Science,Zhejiang University,Hangzhou 310058,China [2]Temasek Life Sciences Laboratory and Department of Biological Science,National University of Singapore,Singapore 117543,Singapore [3]Biomarker Technologies Corporation,Beijing 101300,China [4]Department of Genebank,Leibniz institute of Plant Genetics and Crop Plant Research,06466 Stadt Seeland,Germany [5]Institute of Crop and Nuclear Agricultural Sciences,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China [6]Office of Research,Innovation & Commercialization,PMAS-Arid Agricultural University Rawalpindi,46300 Rawalpindi,Pakistan [7]Institute of Crop Science,Jinhua Academy of Agncultural Sciences,Jinhua 321017,China

出　　处：《Molecular Plant》2019年第1期30-43,共14页分子植物（英文版）

基　　金：the National Key Basic Research Project (no. 2015CB150205);Natural Science Foundation of China,China (no. 31671597,31370313,31670283);Sino-German Science Center for Research Promotion,China (GZ 1099);Jiangsu Collaborative Innovation Center for Modern Crop Production,China,and the Singapore National Research Foundation Investigatorship Program,Singapore (NRF-NRFI2016-02).

摘　　要：Rapeseed (Brassica napus),an important oilseed crop,has adapted to diverse climate zones and latitudes by forming three main ecotype groups,namely winter,semiwinter,and spring types. However,genetic variations underlying the divergence of these ecotypes are largely unknown. Here,we report the global pattern of genetic polymorphisms in rapeseed determined by resequencing a worldwide collection of 991 germplasm accessions.A total of 5.56 and 5.53 million singlenucleotide polymorphisms (SNPs)as Well as 1.86 and 1.92 million InDels were identified by mapping reads to the reference genomes of "Darmor-bzh"and "Tapidor,"respectively.We generated a map of allelic drift paths that shows splits and mixtures of the main populations,and revealed an asymmetric evolution of the two subgenomes of B.napus by calculating the genetic diversity and linkage disequilibrium parameters.Selective-sweep analysis revealed genetic changes in genes orthologous to those regulating various aspects of plant development and response to stresses.A genome-wide association study identified SNPs in the promoter regions of FLOWERING LOCUS T and FLOWERING LOCUS C orthologs that corresponded to the different rapeseed ecotype groups. Our study provides important insights into the genomic footprints of rapeseed evolution and flowering-time divergence among three ecotype groups,and will facilitate screening of molecular markers for accelerating rapeseed breeding.

关 键 词：Brassica NAPUS genome RESEQUENCING selective sweep ECOTYPE DIVERGENCE GWAS floweringtime trait 

分 类 号：Q[生物学]