短棒状杆菌细胞壁对膀胱癌细胞抑制效果的检验  

Inhibitory effect of Corynebacterium parvum cell wall on bladder cancer cells

作　　者：石铭 张琼文 张静[3] 李婉霞 邵小光 Shi Ming;Zhang Qiongwen;Zhang Jing;Li Wanxia;Shao Xiaoguang(Clinical Laboratory,Dalian Municipal Women and Children's Medical Center Laboratory,Dalian 116037,China;Tianjin Medical University Graduate School,Tianjin 300070,China;Clinical Laboratory,Dalian Municipal Center Hospital,Dalian 116033,China;Department of Medical Laboratory,Dalian University School of Medicine,Dalian 116622,China)

机构地区：[1]大连市妇女儿童医疗中心检验科,116037 [2]天津医科大学研究生院,300070 [3]大连市中心医院检验科,116033 [4]大连大学医学院医学检验系,116622

出　　处：《中国医师进修杂志》2019年第2期97-100,共4页Chinese Journal of Postgraduates of Medicine

摘　　要：目的检验短棒状杆菌细胞壁提取物对膀胱癌细胞的抑制效果。方法将3组大鼠分别膀胱灌注短棒状杆菌细胞壁提取物、致癌剂MNU、0.9%氯化钠,灌注9周后提取每组的大鼠膀胱RNA,检测RNA的纯度与浓度。将提取的RNA反转录成cDNA,设计引物,聚合酶链式反应(PCR)扩增大鼠干扰素/维甲酸诱导凋亡相关基因19(Grim19)、Stat3、β-actin基因。然后对PCR产物进行琼脂糖凝胶电泳检测,用Image J软件对电泳图进行分析。结果从短棒状杆菌提取物组、致癌剂甲基-亚硝基脲(MNU)组、0.9%氯化钠对照组的大鼠膀胱提取到的RNA浓度分别为53.3、93.4和64.9 ng/μl,OD260/OD280在2.03~2.07。琼脂糖电泳结果表明短棒状杆菌细胞壁提取物组的大鼠膀胱中Grim19基因、Stat3基因均高表达,致癌剂MUN组的大鼠膀胱Grim19基因的表达含量低,Stat3基因表达含量高。而0.9%氯化钠对照组Grim19基因、Stat3基因均正常表达。结论在患膀胱癌的大鼠体内,膀胱内灌注的短棒状杆菌细胞壁提取物可以有效地提高抑癌基因Grim19基因的表达,同时降低癌基因Stat3基因的表达,表明短棒状杆菌细胞壁提取物是一种潜在的抗膀胱癌药物。Objective To test the inhibitory effect of Corynebacterium cell wall extract on bladder cancer cells.Methods The bladder RNA was extracted from bladder cancer rats,and concentration and purity of RNA was detected. The extracted RNA was reversely transcribed into cDNA,then the primers were designed and the Grim19 gene,β-actin gene,Stat3 gene was amplified. Finaly the PCR product was subjected to agarose gel electrophoresis.Results OD260 was 0.07,and OD260/OD280 was 2.03 in the group of Corynebacterium parvm extract(3-5# );OD260 was 0.12,and OD260/OD280 was 2.07 in the group of MNU(6-5#);OD260 was 0.08,and OD260/OD280 was 2.07 in the group of physiological saline(7-5#) . The results of agarose gelelectrophoresis showed that Grim19 gene and Stat3 gene was expressed highly in the bladder of rats from the group of CP cell wall extract (3-5# ). Grim19 gene was expressed lowly,while Stat3 gene was expressed highly in the bladder of rats from the group of MNU (6-5#). Grim19 gene and Stat3 gene was expressed normally in the bladder of rats from physiological saline(7-5).Conclusions The expression situation of antitumor gene Grim19 and tumor gene Stat3 in the bladder of rats was inhibited by the cell wall extract of Corynebacterium parvm,which indicates that the cell wall extract of Corynebacterium parvm has inhibitory effect on bladder cancer cells.

关 键 词：细胞壁 膀胱肿瘤 短棒状杆菌 大鼠 

分 类 号：R737.14[医药卫生—肿瘤]