红花油体体外稳定性的研究  

Research on Stability of Oil Bodies in Vitro from Carthamus tinctorious.L

作　　者：杨晶[1,2] 卢震[1] 李一心 王冬月 郑洁 杜林娜[1] 邓思楠 YANG Jing;LU Zhen;LI Yixin;WANG Dongyue;ZHENG Jie;DU Linna;DENG Sinan(College of Life Sciences,Jilin Agricultural University,Changchun 130118,China;Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学生命科学学院,长春130118 [2]吉林农业大学生物反应器与药物开发教育部工程研究中心,长春130118

出　　处：《吉林农业大学学报》2019年第1期48-54,共7页Journal of Jilin Agricultural University

基　　金：长春市科技局重大科技攻关项目(2014077);国家自然科学基金项目(31801396);吉林省教育厅"十三五"科学技术研究项目(2016180);吉林省优秀青年人才基金项目(20170520035JH)

摘　　要：油体属于储藏脂肪的亚细胞单位,它的表面覆盖一层磷脂和油体蛋白,具有非常稳定的结构。文章以红花油体为研究对象,从PBS、NaOH、ddH2O、Tricine和Tris-HCl中筛选出最适合红花油体的提取溶剂并且制备了红花油体悬浮液,考察了不同条件下的红花油体悬浮液体外稳定性。结果表明:通过分析红花油体提取率、粒径和电位,确定PBS为红花油体最佳提取溶剂,在提取的过程中对不同料液比进行比较,确定红花油体提取的最佳料液比为1∶10。同时,考察了pH、盐离子浓度、表面活性剂、处理温度对油体体外稳定性的影响,确定了油体在体外稳定存在的pH为8,盐离子浓度为100 mmol/L,咪唑啉阳离子表面活性剂对红花油体悬浮液稳定效果较好,在合适的pH、盐离子浓度和表面活性剂存在的条件下红花油体悬浮液可以在一定时间内保持稳定存在。The oil body is subcellular organelle for storing fat. Its surface is covered with a layer of phospholipids and oleosins, and its structure is stable. The oil body from Carthamus tinctorious.L was taken as experimental material, and the optimal extraction solvent was selected from PBS, NaOH, ddH2O, Tricine and Tris-HCl. The oil body suspension liquid was prepared. The stability of oil body in vitro was investigated in different conditions. Results showed that PBS was determined to be the best extraction solvent by the analysis of extraction ratio, particle size and electric potential. The optimum solid-liquid ratio was determined to be 1∶10 by the comparison of different solid-liquid ratios during extraction process. The effects of pH value, salt ion concentration, surfactant and treatment temperature on the stability of the oil body in vitro were investigated. The pH value was 8. The salt ion concentration was 100 mmol/L. The tetrahydroglyoxaline cationic surfactant had a better stabilizing effect on the oil body suspension of Carthamus tinctorious.L. The oil body suspension of Carthamus tinctorious.L can remain stable for a certain period of time with a suitable pH, salt ion concentration and presence of a surfactant.

关 键 词：红花 油体 提取溶剂 稳定性 

分 类 号：R284.2[医药卫生—中药学]