α-亚麻酸对TM3细胞活性及相关基因表达的影响  

Effects of ALA on Mice TM3 Leydig Cell Viability and Relative Gene Expression

作　　者：李万宏 李发弟[1,2] 翁秀秀 乐祥鹏[1,2] 李纯锦 LI Wanhong;LI Fadi;WENG Xiuxiu;YUE Xiangpeng;LI Chunjin(College of Pastoral Agriculture Science and Technology,State Key Laboratory of Grassland Agro-e- cosysterns,Lanzhou University,Lanzhou 730020,China;Institute of Ruminant,College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China;College of Animal Science,Jilin University,Changchun 130062,China)

机构地区：[1]兰州大学草地农业科技学院草地农业生态系统国家重点实验室,兰州730020 [2]兰州大学草地农业科技学院反刍动物研究所,兰州730020 [3]吉林大学动物科学学院,长春130062

出　　处：《吉林农业大学学报》2019年第1期102-107,共6页Journal of Jilin Agricultural University

基　　金：国家自然科学基金项目(31702112);兰州大学中央高校基本科研业务费专项资金项目(lzujbky-2015-248);甘肃省青年科技基金项目(1606RJYA245)

摘　　要：α-亚麻酸(ALA)是哺乳动物必需脂肪酸的重要成员,在机体生长和发育过程中起着重要作用。在雄性动物生殖系统,ALA通过代谢生成二十二碳六烯酸(DHA)和二十碳五烯酸(EPA),进而对生殖系统发挥调控功能。采用WST-1、JC-1以及RT-PCR等方法研究ALA对体外培养的小鼠睾丸间质细胞TM3细胞的活力及相关基因表达的影响。结果表明:培养体系中添加10,50,100,200,400μmol/L的ALA,处理24 h可以显著提高TM3细胞活力(P<0.05),并且呈剂量依赖性。与对照组相比,50μmol/L的ALA处理24 h可以促进TM3细胞增殖,提高线粒体膜电位,抑制凋亡相关基因Caspase-3表达(P<0.05),促进类固醇代谢相关基因StAR表达(P<0.05)。这说明在体外培养体系中添加ALA可以增加TM3细胞活性,促进细胞增殖和StAR基因表达,抑制Caspase-3基因表达。As one of the essential fatty acids, α-linolenic acid(ALA) plays an important role in the growth and development of the body. In the male reproductive system, ALA can convert into docosahexaenoic acid and eicosapentaenoic acid, and contribute to male fertility. The present study was undertaken to elucidate direct ALA effects on mice TM3 Leydig cell viability and gene expression using WST-1, JC-1 and real time PCR techniques. The results showed that the addition of different concentrations of ALA(10, 50, 100, 200 and 400 μmol/L) in the culture system for 24 h significantly up-regulated TM3 cell viability in a dose-dependent manner(P<0.05). Meanwhile, the mitochondrial membrane potential was remarkably boosted after being treated with 50 μmol/L ALA for 24 h. ALA also inhibited apoptosis related gene Caspase-3 expression(P<0.05) and stimulated steroid metabolism related gene StAR expression(P<0.05). To conclude, the present study demonstrated that ALA increased mice TM3 Leydig cell viability and stimulated proliferation and StAR expression, however, inhibited Caspase-3 expression.

关 键 词：Α-亚麻酸 TM3细胞 增殖 

分 类 号：S814.1[农业科学—畜牧学] Q492.4[农业科学—畜牧兽医]