非特殊型浸润性乳腺癌患者造血祖细胞激酶1表达与超声征象的关系及其机制  

Correlation of ultrasound characteristics of breast cancer and hematopoietic progenitor kinase 1 expression in patients with invasive ductal breast carcinoma-not otherwise specified and its mechanism

作　　者：王娇娇[1] 宋瑞鹏[2] 董刚[1] 齐清华[1] 李燕[1] Wang Jiaojiao;Song Ruipeng;Dong Gang;Qi Qinghua;Li Yan(Department of Ultrasound, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China;Department of Orthopaedics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China)

机构地区：[1]郑州大学第一附属医院超声影像科,450052 [2]郑州大学第一附属医院骨科,450052

出　　处：《中华实验外科杂志》2019年第2期304-307,共4页Chinese Journal of Experimental Surgery

摘　　要：目的 探讨乳腺癌超声征象与造血祖细胞激酶1(HPK1)表达的相关性。方法 分析经手术病理证实为非特殊型浸润性乳腺癌(IDC-NOS)75例患者术前资料的超声参数:肿块的形态、边缘、后方回声、微小钙化、内部回声及腋窝淋巴结。采用Western blot法检测HPK1在患者癌组织及配对癌旁组织中的表达,并分析其表达与超声参数的关系。选取乳腺癌细胞株MDA-MB-231和MCF-7,构建慢病毒pCDH-HPK1-puro重组载体。重组慢病毒感染乳腺癌细胞株,获得HPK1过表达的乳腺癌稳定细胞株(过表达组),以感染空载体病毒及未处理的细胞株作为阴性对照组、空白组。分别采用Western blot、反转录-聚合酶链反应(RT-PCR)检测这6组细胞HPK1的表达水平。利用划痕愈合实验和Transwell实验检测MDA-MB-231和MCF-7两株细胞株中6组细胞的迁移和侵袭能力。结果 与癌旁组织(0.767±0.141)比较,HPK1蛋白在乳腺癌组织中低表达(0.349±0.058,P<0.05),HPK1的表达与腋窝淋巴结转移的超声征象负相关(P<0.01,r=-0.385),与肿块形态、边缘、后方回声、微小钙化、内部回声等无明显相关(P>0.05)。与阴性对照组、空白组比较,过表达组中HPK1蛋白及mRNA的表达水平上调(P<0.01),细胞的迁移、侵袭能力下降(P<0.05)。结论 HPK1在乳腺癌组织及细胞株中低表达,HPK1过表达可抑制乳腺癌细胞侵袭和迁移。Objective To explore the association between the ultrasound (US) characteristics of breast cancer and hematopoietic progenitor kinase 1 (HPK1) expression in patients with invasive ductal breast carcinoma-not otherwise specified (IDC-NOS).Methods Analysis of ultrasound parameters of preoperative ultrasound retention in 75 patients with IDC-NOS confirmed by surgical pathology. The morphology, margin, posterior echo, microcalcification, internal echo and axillary lymph nodes were observed with conventional US. The expression of HPK1 in IDC-NOS tissue and adjacent tissue was detected by Western blotting, and the relationship between HPK1 expression and these ultrasound parameters was analyzed. The breast cancer cell lines MDA-MB-231 and MCF-7 were selected. A lentiviral pCDH-HPK1-puro recombinant vector was constructed. The recombinant lentivirus-infected breast cancer cell line was obtained, and the HPK1 over-expression breast cancer stable cell line (over-expression group) was obtained, and the cell line infected with the empty vector virus and the untreated group was used as a negative control group and a blank group. The expression level of HPK1 in 6 groups of cells was detected by Western blotting and reverse transcriptase-polymerase chain reaction (RT-PCR). Cell migration and invasion ability of HPK1 in 6 groups of cell lines were detected by scratch healing test and Transwell test.Results Compared with adjacent tissue (0.767±0.141), HPK1 protein was down-regulated in breast cancer tissue (0.349±0.058, P<0.05), and HPK1 expression was negatively correlated with ultrasound signs of axillary lymph node metastasis (r=-0.385). There was no significant correlation between HPK1 and mass morphology, margin, posterior echo, and microscopic calcification or internal echo (P>0.05). Compared with the negative control group and the blank group, the expression level of HPK1 protein and mRNA was up-regulated in the over-expressing group (P<0.01), and the migration and invasion ability of the cells was decreased (P<0.05).Co

关 键 词：非特殊型浸润性乳腺癌 超声征象 造血祖细胞激酶1 分子机制 

分 类 号：R737.9[医药卫生—肿瘤] R445.1[医药卫生—临床医学]