结直肠癌肝转移组织中SIRT2蛋白的表达及其临床意义  

SIRT2 expression in colorectal cancer patients with liver metastasis and its clinical significance

作　　者：王搏[1] 申占龙[1] 叶颖江[1] 姜可伟[1] 王杉[1] Wang Bo;Shen Zhanlong;Ye Yingjiang;Jiang Kewei;Wang Shan(Department of Gastroenterological Surgery,Peking University People′s Hospital,Beijing 100044,China)

机构地区：[1]北京大学人民医院胃肠外科外科肿瘤研究室结直肠癌诊疗研究北京市重点实验室,100044

出　　处：《中华普通外科杂志》2019年第1期49-52,共4页Chinese Journal of General Surgery

基　　金：国家自然科学基金资助项目(81702354、81672375).

摘　　要：目的研究SIRT2蛋白在结直肠癌肝转移组织中的表达及其临床意义。方法收集2010—2012年北京大学人民医院25例同时性结直肠癌肝转移患者肿瘤原发组织、肝转移组织和60例结直肠癌患者肿瘤原发组织及其癌旁正常黏膜组织,应用免疫组织化学方法检测SIRT2蛋白的表达,分析其与临床病理指标、预后的关系,Transwell实验评估细胞的侵袭能力,qRT-PCR法检测E-cadherin和MMP2的mRNA表达。结果与原发灶肿瘤组织相比,结直肠癌患者肝转移组织中SIRT2蛋白低表达(3.6±0.5比6.5±0.8,t=15.37,P<0.01);SIRT2蛋白在结直肠癌组织中表达低于癌旁正常组织(3.1±0.6比5.8±0.4,t=29,P<0.01);肿瘤分化程度高的患者SIRT2蛋白表达高于肿瘤分化程度低的患者,差异具有统计学意义(χ2=7.475,P=0.024)。SIRT2蛋白低表达结直肠癌患者的平均生存时间短于高表达患者[(42.5±6.0)个月比(60.5±5.4)个月],差异有统计学意义(χ2=5.493,P=0.019)。过表达SIRT2蛋白后结肠癌细胞系SW480的侵袭能力显著减弱。结论SIRT2蛋白在结直肠癌肝转移灶和癌组织中低表达,并影响结肠癌细胞的侵袭能力,是影响结直肠癌患者预后的独立因素。Objective To investigate SIRT2 expression and its significance in colorectal cancer liver metastasis. Methods The expression level of SIRT2 was detected in 25 cases of colorectal cancer tissues and corresponding liver metastasis tissues and another 60 cases of colorectal cancer tissues and corresponding adjacent normal tissues by immunohistochemical method.The clinicopathologic correlations of SIRT2 expression were performed using chi-square test.Kaplan-Meier method was used to evaluate the overall survival.Transwell method was used to evaluate invasiveness of SW480 cell line.qRT-PCR was applied to assess expression level of E-cadherin and MMP2. Results SIRT2 expression significantly decreased in colorectal cancer liver metastases (3.6±0.5 vs.6.5±0.8,t=15.37,P<0.01).The low expression level of SIRT2 was significantly associated with tumor differentiation (χ2=7.475,P=0.024).Patients with low SIRT2 expression had a shorter overall survival than those with high SIRT2 expression [(42.5±6.0)mon vs.(60.5±5.4)mon,χ2=5.493,P=0.019].Multivariate analysis showed that SIRT2 expression and distant metastasis were the two factors that were independently associated with prognosis.Overexpression of SIRT2 significantly weakened the invasive ability of SW480 cell line. Conclusion Downregulation of SIRT2 predicts poor prognosis of patients with colorectal cancer for enhancing tumor cell invasive ability.

关 键 词：结直肠肿瘤 肿瘤转移 预后 

分 类 号：R735.34[医药卫生—肿瘤]