TNFα和IL-10在巨细胞病毒感染人胚肺成纤维细胞中的作用  

Effects of TNFα and IL-10 on cytomegalovirus infection in human embryonic lung fibroblasts

作　　者：江振友[1] 曹艳英[2] 林晨[1] 

机构地区：[1]暨南大学医学院微生物学与免疫学教研室,广东广州510632 [2]暨南大学组织移植与免疫实验中心,广东广州510632

出　　处：《中国病理生理杂志》2002年第3期265-268,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目 (No 3960 0 0 0 5);广东省医学科研基金资助项目 (No A1 9992 80 )

摘　　要：目的 :研究TNFα和IL - 1 0对人巨细胞病毒 (HCMV)感染人胚肺成纤维细胞 (HEL)的影响 ,以及感染细胞产生TNFα的变化 ,探讨有关细胞因子在HCMV感染免疫中的作用。方法 :用不同浓度的TNFα和IL - 1 0作用于HEL ,2 4h后感染病毒 ,研究TNFα和IL - 1 0对HCMV感染HEL的影响。用HCMV感染HEL ,感染后 (4、2 4、48、72、96h)分别收集培养上清液 ,用ELISA法检测TNFα含量。结果 :在病毒感染前 ,中、高浓度TNFα或低、中浓度IL - 1 0可抑制HCMV在HEL内的增殖。HCMV感染HEL后 ,细胞产生TNFα的量无明显改变。结论 :TNFα和IL - 1 0在HCMV感染免疫过程中起作用。AIM: To study effects of tumour necrosis factor alpha(TNFα) and interleukin-10(IL-10) on human cytomegalovirus AD169 (HCMV AD169) infection in human embryonic lung fibroblasts (HEL),and the ability of the infected HEL to produce TNFα. We have attempted to understand the effect of cytokines in the immune of HCMV infection. METHODS: TNFα?IL-10 were added separately with different concentrations before 24 h HCMV infection of HEL to study the effect of TNFα and IL-10 on multiplication of HCMV. HCMV was incubated with HEL, TNFα in culture supernatants were measured at 4, 24, 48, 72, 96 h postinfection. The level of TNFα was measured by enzyme-linked immunosorbent assay( ELISA). RESULTS: The addition of TNFα with the concentration of 10-100 μg/L or IL-10 with the concentration of 1-10 μg/L before 24 h HCMV infection of HEL could remarkably inhibited the multiplication of HCMV in HEL. Level of TNFα didn't increase in infected cell supernatants. CONCLUSION: TNFα and IL-10 play an important role in the immune of HCMV infection.

关 键 词：巨细胞病毒 细胞活素类 肺成纤维细胞 TNFΑ IL-10 

分 类 号：R563.13[医药卫生—呼吸系统]