结肠癌细胞通过Fas/FasL诱导淋巴细胞发生凋亡  

Apoptosis of lymphocytes Induced by Fas/FasL of Colon Cancer Cells

作　　者：朱强[1] 邓长生[1] 

机构地区：[1]武汉大学中南医院消化内科,湖北武汉430071

出　　处：《癌症》2002年第3期272-275,共4页Chinese Journal of Cancer

摘　　要：背景与目的:研究发现,肿瘤组织中Fas配体阳性表达区肿瘤浸润淋巴细胞的凋亡率比FasL阴性区高,推测肿瘤细胞可通过增加表达FasL来杀伤肿瘤浸润淋巴细胞。本研究拟在体外验证结肠癌细胞SW480是否可以诱导淋巴细胞发生凋亡。方法:结肠癌细胞系SW480(效应细胞)与对Fas介导凋亡敏感的Jurkat细胞(靶细胞)共培养,分为3种效靶比20∶1,10∶1,5∶1。生长曲线法、流式细胞术、荧光显微镜观察分别检测Jurkat细胞的凋亡情况。结果:流式细胞仪检测结果显示,SW480接种浓度为0(对照)、0.5×105/ml、1×105/ml、2×105/ml时,Jurkat细胞凋亡率为(1.8±0.21)%、(5.49±0.17)%、(11.18±0.14)%、(18.22±0.11)%。荧光显微镜观察结果表明,SW480接种浓度为0(对照)、0.5×105/ml、1×105/ml、2×105/ml时,Jurkat细胞凋亡率为(1.58±0.12)%、(5.22±0.13)%、(9.74±0.21)%、(19.33±0.18)%。3种效靶比条件下,SW480细胞均能诱导Jurkat细胞发生凋亡。随着SW480接种浓度的升高及共培养作用时间的延长,Jurkat细胞凋亡率逐渐增加。每个实验组与对照组相比,P值均小于0.01。结论:结肠癌细胞SW480可以通过Fas系统诱导淋巴细胞发生凋亡,这为结肠癌的免疫逃逸、反击机制提供了又一证据。Background &Objective:It has been reported that apoptotic r ate of tumor infiltrating lymphocytes(TIL)in Fas ligand(FasL)positive region was higher than in FasL negative region,which indicated that tumor cells could kill tumor infiltrating lymphocytes through h igh-expressed FasL.This study was d esigned to determine whether colon c ancer cells SW480could induce apoptosis of lymp hocytes by Fas system in vitro.Methods:Colon cancer cells SW480were co-cul tured with Jurkat cells with different effector-target(E-T)ratios of 20∶1,10∶1,5∶1.Growth cur ve method,flow cytometry(FCM)analysis,fluorescence microscope were used to detect apoptosis of Jurk at cells.Results:Flow cytometry revealed that the apoptotic rate of Jurkat cells were 1.8±0.21,5.49±0.17,11.18±0.14,and 18.22±0.11with the SW480planting concentrations at 0(control),0.5×10 5 /ml,1×10 5 /ml,2×10 5 /ml,respectively.Fluorescence mi croscopy showed that the apoptotic rate of Jurkat cells were 1.58±0.12,5.22±0.13,9.74±0.21,an d 19.33±0.18with the SW480planting concentrations at 0(control),0.5×10 5 /ml,1×10 5 /ml,and 2×10 5 /ml,respectively.Based on the thre e different E-T ratios,SW480cell could all induce a poptosis of Jurkat.Furthermore,Ju rkat cell apoptosis rate increased w ith the increase of planting concentration of SW480a nd co-culture time.There were signi ficant difference between each test group and control(All values P<0.01).Conclusion:Colon cancer cells SW480could induce apoptosis of lymp hocytes throught Fas system,which providing theoretica l foundation for metastasis and counterattack of colon cancer.

关 键 词：结肠癌细胞 JURKAT细胞 细胞共培养 细胞凋亡 FAS系统 FAS/FASL 淋巴细胞 

分 类 号：R735.3[医药卫生—肿瘤]