端粒酶基因在乳腺导管不典型增生中的表达  

Expression of telomerase genes in mamary atypical ductal hyperplasia

作　　者：宋敏[1] 米小轶[1] 李柏林[2] 朱继江[1] 高英贤[1] 崔爽[1] 宋继谒[1] 

机构地区：[1]中国医科大学基础医学院病理学教研室,沈阳110001 [2]中国医科大学基础医学院化学教研室,沈阳110001

出　　处：《中华病理学杂志》2002年第1期30-33,共4页Chinese Journal of Pathology

基　　金：辽宁省自然科学基金资助项目 (0 0 2 0 3 7)

摘　　要：目的　探讨端粒酶基因与乳腺导管不典型增生恶性转化的关系及其作用。方法　应用原位杂交方法检测端粒酶基因hTR和hTRT在 5 0例乳腺导管增生组织 (其中单纯性增生 6例 ,轻度不典型增生 9例 ,中度不典型增生 12例、重度不典型增生 2 3例 )及 2 6例乳腺癌中的表达。结果　在乳腺导管单纯性增生组织中hTR、hTRTmRNA呈弱表达 (1/6 )和阴性。在乳腺导管轻、中度不典型增生组织中hTR、hTRTmRNA呈弱表达 (2 /9、1/9;4 /12、3/12 ) ,在乳腺导管重度不典型增生组织中hTR、hTRTmRNA表达增强 (14 /2 3,6 0 9% ,12 /2 3,5 2 1% ) ,在乳腺癌组织中端粒酶基因hTR、hTRTmRNA呈较强阳性表达 (2 3/2 6 ,88 5 % ;2 1/2 5 ,80 8% )。乳腺导管重度不典型增生组织中的端粒酶基因表达与在轻、中度不典型增生、浸润性导管癌组织中的表达比较差异有显著性意义 (P <0 0 5 ) ,但与导管内癌表达差异无显著性意义 (P >0 0 5 )。结论　端粒酶基因hTR、hTRT的表达与乳腺导管不典型增生细胞的恶性转化密切相关 。Objective To investigate the relationship of telomerase genes and the malignant transformation of atypical mammary ductal hyperplasia. Methods Telomerase genes hTR and hTRT in 50 cases of mammary hyperplasia (the cases included 6 benign hyperplasia, 9 mild atypical hyperplasia, 12 medium atypical hyperplasia, 23 severe atypical hyperplasia) and 26 cases of breast carcinoma were detected by in situ hybridization Results The expression of hTR and hTRT mRNA were weak or negative in benign hyperplasia (1/6,0), weaker in mild moderate atypical hyperplasia (2/9, 1/9, 4/12, and 3/12), strong in severe atypical hyperplasia (14/23, 60 9% and 12/23, 52 1%), while very strong expression (23/26, 88 5% and 21/25, 80 8%) in carcinoma of the breast The difference between mild moderate atypical hyperplasia, invasive ductal carcinoma and severe atypical hyperplasia was significant ( P <0 05) and the difference between severe atypital hyperplasia and intraductal carcinoma was not significant ( P >0 05) Conclusions Telmerase genes (hTR, hTRT) expression is closely related to the malignant transformation of atypical hyperplasia. The reactivated telomerase may play a crucial role in the development of breast cancer

关 键 词：乳腺纤维囊性病 乳腺肿瘤 端粒 末端转移酶 端粒酶 乳腺导管不典型增生 基因表达 

分 类 号：R361[医药卫生—病理学]