食管腺癌组织中p53、p16和PCNA的表达及其临床意义  

Expression of p53,p1 6and PCNA in esophageal adenocarcinoma and its clinical significance

作　　者：叶萍[1] 李兆申[1] 马大烈[2] 倪灿荣[2] 富克远[2] 

机构地区：[1]第二军医大学长海医院消化内科,上海200433 [2]长海医院病理科

出　　处：《第二军医大学学报》2002年第2期173-175,共3页Academic Journal of Second Military Medical University

基　　金：上海市卫生系统百名跨世纪优秀学科带头人培养计划基金资助项目 (98BR0 2 6);上海市青年科技启明星计划资助项目(99QB1 4 0 4 5)

摘　　要：目的 :探讨 p5 3、p16和 PCNA的表达在食管腺癌中的临床生物学意义。 方法 :采用免疫组织化学法 ,检测 30例食管腺癌黏膜组织中 p5 3、p16和 PCNA的表达 ,2 0例正常人的食管组织作为正常对照。结果 :p5 3在食管腺癌黏膜组织中的阳性表达率为 5 6 .7% ,较正常对照组 (10 .0 % )明显增高 ;p16在食管腺癌黏膜组织中的阳性表达率为 36 .7% ,较正常对照组 (5 0 .0 % )降低 ;PCNA在食管腺癌黏膜组织较正常食管表达强度和阳性表达率明显增强 ,统计学处理均有明显差异 (P <0 .0 1或 P<0 .0 5 ) ,表达强度在食管腺癌组以 ( )～ ( )多见 ,而正常对照组则多为 (- )～ (+)。食管腺癌患者三项基因的表达至少有一项呈现为阳性。 结论 :p5 3、p16和 PCNA在食管腺癌的发生发展过程中起重要作用 ,其蛋白表达对估价食管腺癌具有重要意义。Objective:To explore the relationship between the expression of p5 3,p16 and PCNA and the clinicobiological behavior of human esophageal adenocarcinoma. Methods:The products of the genes of p5 3,p16 and PCNA were detected using im munohistochemistry in 30 cases of esophageal adenocarcinom as,and 2 0 cases were detected as norm al control group. Results:It was showed that the positive rate and staining intensity of the p5 3,p16 and PCNA gene expressions in esophageal adenocarcinom a were higher than those in norm al esophageal tissues (P<0 .0 5 ) . p5 3and p16 gene expression was 5 6 .7% and 36 . 7% respectively in esophageal adenocarcinoma. While an evaluation of proliferation with expression of PCNA gene,the positive rate was 83. 3% with esophageal adenocarcinomas. Adenocarcinom a tissue were stained with positive reaction for at least one- marker. Conclusion:p5 3,p16 and PCNA,as the useful biom arker of tumor, play im portant roles in the formation and development of esophageal adenocarcinom a. [

关 键 词：食管肿瘤 食管腺癌 P53基因 P16基因 PCNA 基因表达 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤]